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首页> 外文期刊>Infection and immunity >Molecular analysis of a sphingomyelinase C gene from Leptospira interrogans serovar hardjo.
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Molecular analysis of a sphingomyelinase C gene from Leptospira interrogans serovar hardjo.

机译：来自问号钩端螺旋体血清型硬种的鞘磷脂酶C基因的分子分析。

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A thermolabile hemolysin from Leptospira interrogans serovar hardjo, strain Sponselee, was shown to specifically degrade sphingomyelin. Nucleotide sequence determination revealed that sphingomyelinase activity was encoded by an open reading frame of 1,668 nucleotides. Although a putative signal sequence could be identified, no evidence for protein export in either L. interrogans or Escherichia coli was obtained. The apparent molecular mass of the expression product in E. coli minicells was 41.2 kilodaltons, whereas open reading frame 1 encoded a protein of 63,268 daltons. The observed difference may be explained by processing at the carboxy-terminal part of the hemolysin in E. coli. A high degree of similarity on the DNA and protein levels with Staphylococcus aureus beta-hemolysin and sphingomyelinase C from three Bacillus cereus strains was observed. The presence of various sphingomyelinase genes within the L. interrogans species is demonstrated.
机译：研究表明，来自问号钩端螺旋体血清型硬乔的热不稳定溶血素可特异性降解鞘磷脂。核苷酸序列测定表明，鞘磷脂酶活性由1,668个核苷酸的开放阅读框编码。尽管可以鉴定出推定的信号序列，但没有获得询问豆乳杆菌或大肠杆菌中蛋白质输出的证据。在大肠杆菌小细胞中表达产物的表观分子量为41.2千道尔顿，而开放阅读框1编码的蛋白质为63268道尔顿。观察到的差异可以通过在大肠杆菌中的溶血素的羧基末端部分进行处理来解释。观察到与三种金黄色芽孢杆菌菌株的金黄色葡萄球菌β-溶血素和鞘磷脂酶C的DNA和蛋白质水平高度相似。证实了在询问乳杆菌物种内各种鞘磷脂酶基因的存在。

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