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首页> 外文期刊>Infection and immunity >Protection of rat intestine against cholera toxin challenge by monoclonal anti-idiotypic antibody immunization via enteral and parenteral routes.
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Protection of rat intestine against cholera toxin challenge by monoclonal anti-idiotypic antibody immunization via enteral and parenteral routes.

机译：通过肠内和肠胃外途径的单克隆抗独特型抗体免疫，保护大鼠肠道免受霍乱毒素攻击。

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A mouse monoclonal anti-idiotypic (anti-id) immunoglobulin M (IgM) antibody, called MAb2, was raised against a mouse monoclonal anti-cholera toxin (anti-CT) antibody (MAb1). The MAb2 was shown, by competition with CT for MAb1, to bear the internal image of an epitope of CT. MAb2 immunization of rats was performed via the intraperitoneal, intragastric, and intrajejunal routes and compared with immunization of rats with either a control, isotype- and allotype-matched MAb or with CT via the same routes. Both serum IgG and bile IgA anti-CT Ab3's were detected by enzyme-linked immunosorbent assay in anti-id MAb2-immunized rats, although their titers were lower than those in CT-immunized rats. No anti-CT antibodies were detected in sera and bile of rats immunized with the control MAb. When tested for degree of gut protection against a CT challenge, rats immunized with MAb2 by the intrajejunal route showed a rather high degree of protection, which was only slightly lower than that of rats immunized with CT via the same route; all rats but one immunized with the control MAb were unprotected. There was, however, no correlation between serum or bile anti-CT titers and degree of gut protection in MAb2-immunized rats. Their serum anti-CT Ab3's were purified by adsorption and elution from a CT immunosorbent and resembled anti-CT MAb1 in their unique reactivity with MAb2. This constitutes to our knowledge the second report of protection against a pathogen by anti-id immunization via the enteric route.
机译：产生了针对小鼠单克隆抗霍乱毒素（anti-CT）抗体（MAb1）的小鼠单克隆抗独特型（anti-id）免疫球蛋白M（IgM）抗体。通过与CT竞争MAb1，MAb2带有CT表位的内部图像。通过腹膜内，胃内和空肠途径对大鼠进行MAb2免疫，并将其与通过对照，同种型和同种异型匹配的MAb或通过相同途径的CT对大鼠的免疫进行比较。通过酶联免疫吸附法在抗id MAb2免疫的大鼠中检测了血清IgG和胆汁IgA抗CT Ab3，尽管它们的滴度低于CT免疫大鼠。在用对照MAb免疫的大鼠的血清和胆汁中未检测到抗CT抗体。当测试肠抵抗CT攻击的程度时，通过空肠途径用MAb2免疫的大鼠显示出较高的保护程度，仅略低于通过相同途径用CT免疫的大鼠。除一只用对照单克隆抗体免疫的大鼠外，其他所有动物均未受保护。但是，在用MAb2免疫的大鼠中，血清或胆汁抗CT滴度与肠道保护程度之间没有相关性。通过从CT免疫吸附剂中吸附和洗脱来纯化其血清抗CT Ab3's，类似于抗CT MAb1，它们与MAb2具有独特的反应性。据我们所知，这是通过肠道途径进行的抗id免疫方法预防病原体的第二份报告。

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