• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Molecular characterization of an RTX toxin determinant from Actinobacillus suis.
【24h】

Molecular characterization of an RTX toxin determinant from Actinobacillus suis.

机译：猪放线杆菌RTX毒素决定簇的分子表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

RTX cytolysins are a family of calcium-dependent, pore-forming, secreted toxins found in a variety of gram-negative bacteria. The prototypical member of this family is the alpha-hemolysin of Escherichia coli. The RTX genetic determinants from seven members of the family Pasteurellaceae, Pasteurella haemolytica, Actinobacillus actinomycetemcomitans, and A. pleuropneumoniae serotypes 1,5,7, and 9 were previously cloned and sequenced. Using the leukotoxin determinant from P. haemolytica serotype A1 as a probe, we detected the presence of RTX-type determinants in Actinobacillus suis, A. equuli, and A. lignieresii of the family Pasteurellaceae. All three species elaborate proteins of approximately 104 to 110 kDa that are recognized by polyclonal antisera against the 104-kDa hemolysin of A. pleuropneumoniae serotype 1. An RTX determinant of A. suis isolate 3714 was cloned and sequenced and was found to be almost identical to the RTX determinant of A. pleuropneumoniae serotypes 5 and 9. In addition, the determinant is not composed of four contiguous genes, as had been reported for most other RTX determinants; instead, the genes encoding the two proteins responsible for secretion of the toxin are at a locus distinct from that containing the toxin structural and activation genes.
机译：RTX溶血素是在多种革兰氏阴性细菌中发现的钙依赖性，成孔性分泌毒素家族。该家族的典型成员是大肠杆菌的α-溶血素。先前克隆并测序了来自巴斯德氏菌科，溶血巴斯德氏菌，放线放线杆菌，放线杆菌肺炎血清型1、5、7和9的七个成员的RTX遗传决定因素。使用溶血性疟原虫血清型A1的白细胞毒素决定簇作为探针，我们检测到巴斯德氏菌科的猪放线杆菌，马曲霉和轻木曲霉中存在RTX型决定簇。所有这三种物种都合成了约104至110 kDa的蛋白质，这些蛋白质被针对胸膜肺炎链球菌血清型1的104 kDa溶血素的多克隆抗血清所识别。克隆并测序了猪链球菌分离株3714的RTX决定子，发现几乎相同胸膜肺炎链球菌血清型5和9的RTX决定簇。此外，该决定簇不由四个连续基因组成，正如大多数其他RTX决定簇所报道的那样;相反，编码负责毒素分泌的两种蛋白质的基因位于与包含毒素结构和激活基因的基因不同的基因座。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号