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首页> 外文期刊>Infection and immunity >Similar cytokine induction profiles of a novel streptococcal exotoxin, MF, and pyrogenic exotoxins A and B.
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Similar cytokine induction profiles of a novel streptococcal exotoxin, MF, and pyrogenic exotoxins A and B.

机译：新型链球菌外毒素，MF和热原性外毒素A和B的相似细胞因子诱导概况。

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The cytokine production induced by a newly discovered streptococcal exotoxin, MF, and the pyrogenic exotoxins SpeA and SpeB was determined by in vitro stimulation of peripheral blood mononuclear cells (PBMCs) obtained from healthy blood donors. The induction and kinetics of interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-1 receptor antagonist, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-10, gamma interferon, tumor necrosis factor alpha (TNF-alpha), TNF-beta, and granulocyte-macrophage colony-stimulating factor were studied at the single-cell level by use of cytokine-specific monoclonal antibodies and intracellular immunofluorescent juxtanuclear staining. The cytokine-producing cells, with the exception of IL-1-expressing cells, had a characteristic morphology generated by the accumulation of cytokines in the Golgi organelle. MF, SpeA, and SpeB induced a massive gamma interferon and TNF-beta response in 10 to 16% of the PBMCs after 48 to 96 h of cell stimulation. In contrast, IL-2 and TNF-alpha production was detected in only 1 to 3% of the PBMCs. The induction of a lymphocyte TH2 phenotype response, including production of IL-3, IL-4, IL-5, and IL-10, was weak. However, the monokines, IL-1 alpha, IL-1 beta, IL-1 receptor antagonist, and IL-8, were consistently found and gradually produced, peaking at 24 h in approximately 5 to 8% of the PBMCs. MF showed extensive cytokine- and proliferation-inducing capacities equal to those of SpeA and SpeB, which suggests that MF is also a superantigen. A marked interindividual variation could be noted both in the proliferative response and in the cytokine induction of lymphocytes isolated from different individuals, which may be one explanation for the varying clinical severity noticed during group A streptococcal infections.
机译：通过体外刺激获自健康献血者的外周血单核细胞（PBMC），确定了新发现的链球菌外毒素MF和热原性外毒素SpeA和SpeB诱导的细胞因子产生。白介素-1α（IL-1α），IL-1β，IL-1受体拮抗剂，IL-2，IL-3，IL-4，IL-5，IL-6，IL-8的诱导和动力学，IL-10，γ干扰素，肿瘤坏死因子α（TNF-alpha），TNF-β和粒细胞巨噬细胞集落刺激因子在单细胞水平上通过使用细胞因子特异性单克隆抗体和细胞内免疫荧光juxtanuclear进行了研究染色。除了表达IL-1的细胞外，产生细胞因子的细胞具有通过高尔基细胞器中细胞因子的积累而产生的特征形态。 MF，SpeA和SpeB在刺激48至96小时后，在10至16％的PBMC中诱导了大规模的γ干扰素和TNF-β反应。相反，仅在1-3％的PBMC中检测到IL-2和TNF-α的产生。淋巴细胞TH2表型反应的诱导作用很弱，包括IL-3，IL-4，IL-5和IL-10的产生。但是，一贯发现并逐渐产生单种因子IL-1α，IL-1β，IL-1受体拮抗剂和IL-8，在大约5％至8％的PBMC中于24小时达到峰值。 MF显示出与SpeA和SpeB相同的广泛的细胞因子和增殖诱导能力，这表明MF也是超抗原。在从不同个体分离的淋巴细胞的增殖反应和细胞因子诱导中都可以注意到明显的个体间差异，这可能是对A组链球菌感染期间临床严重程度变化的一种解释。

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