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首页> 外文期刊>Infection and immunity >A mannoprotein constituent of Candida albicans that elicits different levels of delayed-type hypersensitivity, cytokine production, and anticandidal protection in mice.
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A mannoprotein constituent of Candida albicans that elicits different levels of delayed-type hypersensitivity, cytokine production, and anticandidal protection in mice.

机译：白色念珠菌的一种甘露糖蛋白成分，在小鼠中引起不同水平的迟发型超敏反应，细胞因子产生和抗念珠菌保护。

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To identify major immunogenic constituents of Candida albicans, the effect of a mannoprotein fraction (MP-F2) on the elicitation of a delayed-type hypersensitivity (DTH) reaction, cytokine production, and protection from a virulent Candida challenge in a mouse candidiasis model was studied. In mice immunized with whole cells of a low-virulence strain of C. albicans and thus protected against a challenge with a highly virulent strain of this fungus, MP-F2 was able to elicit a strong DTH response that was accompanied by splenocyte proliferation in vitro in the presence of Candida antigen. The supernatants of MP-F2-stimulated splenocyte cultures contained gamma interferon (IFN-gamma, a typical CD4+ T helper-1 (Th1) cytokine, but no interleukin-4, (IL-4), a typical CD4+ Th2 cytokine. IFN-gamma was produced by CD4+ cells, and its level could be greatly increased by the addition of anti-IL-4 or, mostly, anti-IL-10 antibodies to the CD4+ cell cultures. Upon a suitable schedule of immunization, MP-F2 was also able to induce a vigorous DTH response in Candida-uninfected mice, a response that could be efficiently transferred into naive recipients by CD4+ cells from the spleens of MP-F2-immunized mice. The immunization described above also conferred to mice a low degree of protection against a virulent Candida challenge, both in terms of median survival time and in the number of Candida cells in the kidney. However, while DTH induction by MP-F2 was as strong as that induced by whole cells, MP-F2-induced protection was significantly weaker than that conferred by Candida whole-cell immunization. Mice immunized with either MP-F2 or Candida whole cells had an inverted ratio between the number of CD4+ splenocytes producing IFN-gamma and that of cells producing IL-4, compared with nonimmunized animals. However, the number of IL-4-producing CD4+ cells was significantly higher in MP-F2-vaccinated, weakly protected mice than in Candida whole-cell-vaccinated, highly protected animals. Overall, our data suggest that the MP-F2 fraction contains one or more major immunogens of C. albicans which are capable of interfering with the balance of CD4+ Th1 and Th2 responses that is so critical in the outcome of host-Candida relationship and are thus potentially relevant in the mechanisms of Candida-specific DTH regulation and protection.
机译：为了鉴定白色念珠菌的主要免疫原性成分，在小鼠念珠菌病模型中，使用甘露糖蛋白组分（MP-F2）对引起迟发型超敏反应（DTH）反应，细胞因子产生以及免受强力念珠菌攻击的保护作用为：研究。在用低毒力的白色念珠菌菌株的全细胞免疫并因此免受这种真菌高毒力菌株攻击的小鼠中，MP-F2能够引发强烈的DTH反应，并伴随体外脾细胞增殖在念珠菌抗原存在下。 MP-F2刺激的脾细胞培养物的上清液含有γ干扰素（IFN-γ，一种典型的CD4 + T辅助物1（Th1）细胞因子，但不含白介素4（IL-4），一种典型的CD4 + Th2细胞因子。 γ是由CD4 +细胞产生的，可以通过向CD4 +细胞培养物中添加抗IL-4抗体（或主要是抗IL-10抗体）来极大地提高其γ水平。还可以在未感染念珠菌的小鼠中诱导强烈的DTH应答，这种应答可以通过MP-F2免疫小鼠脾脏中的CD4 +细胞有效地转移到幼稚受体中。在中位生存时间和肾脏中念珠菌细胞数量方面均能抵抗强力念珠菌攻击，但是，尽管MP-F2诱导DTH的能力与全细胞诱导的强，但MP-F2诱导的保护明显弱于念珠菌全细胞l免疫。与未免疫的动物相比，用MP-F2或念珠菌全细胞免疫的小鼠在产生IFN-γ的CD4 +脾细胞数目与产生IL-4的细胞数目之间具有倒数比。但是，在接种MP-F2的弱保护小鼠中，产生IL-4的CD4 +细胞的数量要比在接种假丝酵母的全细胞的高度保护动物中高得多。总的来说，我们的数据表明，MP-F2部分含有一种或多种白色念珠菌的主要免疫原，它们能够干扰CD4 + Th1和Th2反应的平衡，这在宿主-念珠菌关系的结果中至关重要与念珠菌特异性DTH调节和保护机制潜在相关。

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