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Comparative Estimates of Bacterial Affinities and Adsorption Sites on Hydroxyapatite Surfaces

机译：羟基磷灰石表面细菌亲和力和吸附位点的比较估计

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The adsorption of strains of prominent oral bacteria to hydroxyapatite (HA) surfaces was studied by use of an adsorption model based on the Langmuir adsorption isotherm; this permitted comparative estimates of the number of adsorption sites and the strength of the adsorption bonds on untreated and salivatreated HA surfaces for strain of Streptococcus mutans, S. salivarius, S. sanguis, S. mitis, Actinomyces viscosus, and A. naeslundii. The experimental data closely followed the adsorption model as judged by the high correlation coefficients obtained for all strains studied. Adsorption to untreated HA was similar for strains of the six species studied, suggesting that a common adsorption mechanism, possibly Ca2+ bridging, may exist for attachment to HA. More complex interactions appeared to be involved in bacterial adsorption to saliva-treated HA since adsorption of the strains tested at unsaturating cell concentrations varied more than 30-fold. This indicates that adsorbed salivary components on HA surfaces impart a higher order of specificity for subsequent bacterial adsorption. Fewer cells of strains of S. mutans, S. salivarius, and A. naeslundii adsorbed to saliva-treated HA than to untreated HA because adsorbed salivary components presented fewer adsorption sites. Substantially higher numbers of cells of strains of S. sanguis, S. mitis, and A. viscosus adsorbed to saliva-treated HA because the film of adsorbed salivary components increased the number of adsorption sites for these strains. The affinity constants for all but one strain studied were lower on saliva-treated HA than on untreated HA. The number of bacterial cells which adsorbed to saliva-treated HA more closely related to the number of available binding sites than to the strength of their adsorption bonds when tested at an initial concentration of 2 × 107 organisms/ml. Although some differences were observed in the adsorption of strains of S. mutans representative of five serological groups, the numbers which attached to saliva-treated HA did not vary widely; this suggests that factors other than their ability to attach to a pellicle-covered HA surface may be responsible for their varying geographic distribution in human populations.
机译：利用基于Langmuir吸附等温线的吸附模型，研究了口腔内重要细菌菌株对羟基磷灰石（HA）表面的吸附。这允许比较估计变形链球菌，唾液链球菌，桑氏球菌，链球菌，粘性放线菌菌株在未处理和唾液处理的HA表面上的吸附位点数量和吸附键的强度。以及 A。 naeslundii 。通过对所有研究菌株获得的高相关系数判断，实验数据紧密遵循吸附模型。对这六个物种的菌株，未处理的HA的吸附情况相似，这表明可能存在一个常见的吸附机制，可能是Ca 2 + 桥接，可能会附着在HA上。细菌吸附到唾液处理的HA中似乎涉及更复杂的相互作用，因为在不饱和细胞浓度下测试的菌株的吸附变化超过30倍。这表明HA表面上吸附的唾液成分为随后的细菌吸附赋予更高的特异性。 S菌株的细胞较少。变形链球菌，唾液链球菌（S. salivarius）和 A。 naeslundii 吸附到唾液处理的HA上比未处理的HA吸附，因为被吸附的唾液成分呈现较少的吸附位点。 S菌株的细胞数量要多得多。桑吉斯（Sanguis），米色链球菌（S. mitis）和 A。粘性吸附到唾液处理的HA中，因为唾液成分的吸附膜增加了这些菌株的吸附位点数量。经唾液处理的HA的亲和常数（除一种菌株外）均低于未处理的HA。在初始浓度为2×10 7 生物/次时，吸附到唾液处理的HA上的细菌细胞数量与可用结合位点数量的关系比与其吸附键强度的关系更密切。毫升尽管在 S菌株的吸附中观察到一些差异。代表五个血清学组的变异体，唾液处理的HA附着的数量差异不大;这表明，除了其附着在覆盖有薄膜的HA表面上的能力以外，其他因素也可能是其在人群中地理分布不同的原因。

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