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首页> 外文期刊>Infection and immunity >Characterization of two different antibody specificities recognizing distinct antigenic determinants in free lipid A of Escherichia coli.
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Characterization of two different antibody specificities recognizing distinct antigenic determinants in free lipid A of Escherichia coli.

机译：识别大肠杆菌游离脂质A中不同抗原决定簇的两种不同抗体特异性的表征。

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Antisera were raised in rabbits with acid-treated Re mutant bacteria from Salmonella minnesota and Escherichia coli and tested in a passive hemolysis assay with di- and monophosphorylated free lipid A of E. coli (LipA-Ac and LipA-HCl, respectively) coated onto sheep erythrocytes. Depending on the acid used to prepare the immunogen (acetic versus hydrochloric acid), different antibody specificities were obtained. Antiserum prepared against HCl-treated bacteria was found to react with both antigens to the same extent (i) in the passive hemolysis test, (ii) in the passive hemolysis inhibition test, and (iii) in absorption experiments, suggesting that antibodies in this antiserum recognize an antigenic determinant equally present in LipA-Ac and LipA-HCl. Antiserum raised against acetic acid-treated bacteria reacted with the homologous antigen (LipA-Ac) in the passive hemolysis and passive hemolysis inhibition test as well as in absorption experiments. However, the antiserum failed to react with the heterologous antigen (LipA-HCl) in the hemolysis test and during absorption, whereas in inhibition studies interaction of this antiserum with both antigens was observed. The inhibiting capacity of LipA-Ac was lower compared with that of LipA-HCl, indicating that the antigenic determinant of LipA-Ac is partly expressed by LipA-HCl in solution, but not when fixed on the surface of sheep erythrocytes. The role of glycosidically linked phosphate in lipid A is discussed with respect to antigenicity.
机译：用来自明尼苏达州沙门氏菌和大肠杆菌的经酸处理的Re突变细菌在兔中产生抗血清，并在被动溶血试验中进行测试，将大肠杆菌的二磷酸和单磷酸化游离脂质A（分别为LipA-Ac和LipA-HCl）包被绵羊红细胞。取决于用于制备免疫原的酸（乙酸与盐酸），可获得不同的抗体特异性。发现针对HCl处理的细菌制备的抗血清与两种抗原的反应程度相同（i）在被动溶血试验中，（ii）在被动溶血抑制试验中，以及（iii）在吸收实验中，提示该抗体在抗血清识别同样存在于LipA-Ac和LipA-HCl中的抗原决定簇。在被动溶血和被动溶血抑制试验以及吸收实验中，针对醋酸处理细菌产生的抗血清与同源抗原（LipA-Ac）反应。然而，在溶血试验和吸收过程中，抗血清未能与异源抗原（LipA-HCl）反应，而在抑制研究中，观察到该抗血清与两种抗原的相互作用。 LipA-Ac的抑制能力比LipA-HCl的抑制能力低，表明LipA-Ac的抗原决定簇在溶液中部分由LipA-HCl表达，但当固定在绵羊红细胞表面时则不表达。关于抗原性，讨论了糖基连接的磷酸盐在脂质A中的作用。

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