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首页> 外文期刊>Infection and immunity >Colonization of the mouse intestine by an avirulent Salmonella typhimurium strain and its lipopolysaccharide-defective mutants.
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Colonization of the mouse intestine by an avirulent Salmonella typhimurium strain and its lipopolysaccharide-defective mutants.

机译：无毒鼠伤寒沙门氏菌菌株及其脂多糖缺陷型突变体对小鼠肠道的定殖。

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For study of the role of lipopolysaccharide (LPS) character in colonization of the mouse large intestine, use was made of S. typhimurium strain SL5316, which is streptomycin resistant and smooth (wild-type LPS) but nonvirulent because it is Aro- (aromatic dependent). Several rough variants of different LPS chemotype derived from strain SL5316 comprised: an rfb deletion transductant making type Ra (complete core) LPS; an rfaJ mutant making incomplete core of type Rb2; and an rfa-990 mutant making LPS core less complete than chemotype Rb2. We tested these strains for colon-colonizing ability by feeding them to male CD-1 mice receiving streptomycin sulfate (5 g/liter) in their drinking water. Each strain, if fed alone, was found in the feces throughout the 15 days of the experiment at about 10(8) CFU/g for the smooth strain or 10(7) CFU/g for each of its rough derivatives. However, when mice were fed equal numbers of two strains (with differentiating antibiotic resistance characters), the strain with the more complete LPS was found in the feces in great excess, 1000- to 100,000-fold, according to the pair. Thus, when strains were placed in direct competition with one another, their relevant colon-colonizing abilities were found to be wild type greater than rfb much greater than rfaJ greater than rfa-990, showing that the ability of a Salmonella strain to colonize the mouse large intestine decreases as its LPS structure becomes more defective.
机译：为了研究脂多糖（LPS）特性在小鼠大肠定植中的作用，使用了鼠伤寒沙门氏菌菌株SL5316，该菌株具有链霉素抗性和顺滑性（野生型LPS），但无毒，因为它具有芳香性（芳香族取决于）。源自菌株SL5316的不同LPS化学型的几个粗略变体包括：产生Rafb缺失的转导子，形成Ra（完全核心）LPS型;一个rfaJ突变体，其Rb2型核心不完整;和一个rfa-990突变体，使LPS核心不如化学型Rb2完整。通过将它们喂入饮用水中接受硫酸链霉素（5 g /升）的雄性CD-1小鼠中，我们测试了这些菌株的结肠克隆能力。在整个实验的15天中，如果在粪便中发现每种菌株，如果分别饲喂，则对于平滑菌株而言，约为10（8）CFU / g，对于每种粗衍生物而言，约为10（7）CFU / g。然而，当给小鼠喂食相等数量的两种菌株（具有不同的抗生素抗性特征）时，在粪便中发现具有更完整LPS的菌株，其排泄量大大过量，为1000至100,000倍。因此，当将菌株彼此直接竞争时，发现它们的相关结肠定殖能力是野生型，大于rfb，远大于rfaJ，大于rfa-990，表明沙门氏菌菌株定居小鼠的能力。大肠随着其LPS结构的缺陷而减少。

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