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首页> 外文期刊>Infection and immunity >In vivo colonization of the mouse large intestine and in vitro penetration of intestinal mucus by an avirulent smooth strain of Salmonella typhimurium and its lipopolysaccharide-deficient mutant.
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In vivo colonization of the mouse large intestine and in vitro penetration of intestinal mucus by an avirulent smooth strain of Salmonella typhimurium and its lipopolysaccharide-deficient mutant.

机译：鼠伤寒沙门氏菌无毒的平滑菌株及其脂多糖缺陷型突变体在小鼠大肠的体内定植和肠粘液的体外渗透。

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摘要

The relative abilities of an avirulent Salmonella typhimurium strain with wild-type lipopolysaccharide (LPS) character, SL5319, and a nearly isogenic LPS-deficient mutant, SL5325, to colonize the large intestines of streptomycin-treated CD-1 mice in vivo and to penetrate colonic mucus in vitro were studied. Previously it had been shown that, when fed simultaneously to streptomycin-treated mice (approximately 10(10) CFU each), the S. typhimurium strain with wild-type LPS colonized at 10(8) CFU/g of feces indefinitely, whereas the LPS-deficient mutant dropped within 3 days to a level of only 10(4) CFU/g of feces. In the present investigation, when SL5325 was allowed to colonize for 8 days before feeding mice SL5319 or when it was fed to mice simultaneously with an Escherichia coli strain of human fecal origin (10(10) CFU each), both strains colonized indefinitely at 10(7) CFU/g of feces. Moreover, when the wild-type and LPS-deficient mutant strains were fed to mice simultaneously in low numbers (approximately 10(5) CFU each) the strains survived equally well in the large intestines for 8 days, after which the LPS-deficient mutant was eliminated (less than 10(2) CFU/g of feces), whereas the wild-type colonized at a level of 10(7) CFU/g of feces. In addition although both strains were able to adhere to mucus and epithelial cell preparations in vitro, the wild-type strain was shown to have greater motility and chemotactic activity on CD-1 mouse colonic mucus in vitro and to more rapidly penetrate and form a stable association with immobilized colonic mucosal components in vitro. Based on these data, we suggest that the ability of an S. typhimurium strain to colonize the streptomycin-treated mouse large intestine may, in part, depend on its ability to penetrate deeply into the mucus layer on the intestinal wall and subsequently, through growth, colonize the mucosa.
机译：具有野生型脂多糖（LPS）特性的无毒鼠伤寒沙门氏菌菌株SL5319和近等基因LPS缺陷型突变体SL5325在链霉素处理过的CD-1小鼠的大肠内定植并穿透的相对能力对结肠粘液进行了体外研究。以前的研究表明，当同时喂食链霉素处理的小鼠（每只大约10（10）CFU）时，带有野生型LPS的鼠伤寒沙门氏菌菌株会无限期地以10（8）CFU / g粪便定居，而LPS缺陷突变体在3天内下降至仅10（4）CFU / g粪便的水平。在本研究中，当SL5325在饲喂小鼠SL5319之前先定居8天，或者与人类粪便来源的大肠杆菌菌株（每只10（10）CFU）同时饲喂给小鼠时，两种菌株都在10时无限期地定居。 （7）CFU /克粪便。此外，当将野生型和LPS缺陷型突变株以低数量（每只约10（5）CFU）同时喂给小鼠时，这些菌株在大肠中同样存活8天，此后LPS缺陷型突变株消除（少于10（2）CFU / g粪便），而野生型以10（7）CFU / g粪便定植。另外，尽管两种菌株都能够在体外粘附于粘液和上皮细胞制剂，但野生型菌株在体外对CD-1小鼠结肠粘液具有更大的运动性和趋化活性，并能更快地渗透并形成稳定的与固定的结肠粘膜成分体外结合。基于这些数据，我们认为鼠伤寒沙门氏菌菌株在链霉素处理的小鼠大肠中定殖的能力可能部分取决于其深入渗透到肠壁粘液层并随后通过生长的能力。 ，定居在粘膜上。

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