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首页> 外文期刊>Infection and immunity >Neutrophil activity in abscess-bearing mice: comparative studies with neutrophils isolated from peripheral blood, elicited peritoneal exudates, and abscesses.
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Neutrophil activity in abscess-bearing mice: comparative studies with neutrophils isolated from peripheral blood, elicited peritoneal exudates, and abscesses.

机译：脓肿小鼠的嗜中性粒细胞活性：从外周血，引出腹膜渗出液和脓肿的嗜中性粒细胞的比较研究。

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Intraabdominal abscesses were induced in mice by intraperitoneal inoculation of Bacteroides fragilis and Escherichia coli plus bran as the abscess-potentiating agent. Six- or seven-day-old abscesses were mechanically disaggregated in buffer, and the cells obtained were fractionated on discontinuous Percoll density gradients. Neutrophil populations of different density, each approximately 90% pure, were isolated. When the abscess-derived neutrophils were subsequently incubated with normal serum in vitro under aerobic conditions, the viability of the gram-negative bacteria that had been phagocytosed within the abscess did not change significantly. This anergy to intracellular bacteria (on subsequent incubation in vitro under optimal conditions for phagocytic killing) was also found for neutrophils that had been obtained from abscesses induced by a mixture that included Proteus mirabilis plus B. fragilis and from those induced by E. coli plus P. mirabilis. While unable to significantly kill intracellular organisms that had been phagocytosed in vivo, the abscess-derived neutrophils could engulf and kill organisms to which they were exposed in vitro. Neutrophils from abscesses induced by P. mirabilis only plus bran killed that organism introduced in vitro significantly more effectively than the organisms that had been engulfed in vivo. In contrast, neutrophils from abscesses induced by the gram-positive organism Staphylococcus aureus plus bran were able to kill their intracellular organisms on subsequent incubation in vitro as effectively as they could kill added S. aureus. Neutrophils isolated from the peripheral blood and from induced peritoneal exudates of abscess-bearing mice were able to phagocytose and kill organisms in vitro with greater efficiency than abscess-derived neutrophils. The mechanism whereby neutrophils from abscesses induced by the gram-positive organism S. aureus can kill the organisms phagocytosed in vivo on subsequent in vitro incubation, in contrast to the relative anergy to their intracellular organisms displayed by neutrophils derived from abscesses induced by combinations of gram-negative bacteria, is not known.
机译：腹腔内的脓肿是通过腹腔内接种脆弱的拟杆菌，大肠杆菌和麸皮作为脓肿增强剂而引起的。在缓冲液中机械分解六天或七天大的脓肿，并在不连续的Percoll密度梯度上分级分离获得的细胞。分离了每个密度约为90％的不同密度的嗜中性粒细胞。当脓肿来源的中性粒细胞随后在有氧条件下与正常血清在体外孵育时，在脓肿内被吞噬的革兰氏阴性细菌的活力没有明显改变。还发现细胞嗜中性粒细胞对细胞内细菌无反应（随后在最佳条件下进行吞噬杀死），这些嗜中性粒细胞是由脓毒症获得的，所述脓肿是由脓毒症（包括奇异变形杆菌和脆弱芽孢杆菌）和大肠杆菌（包括大肠杆菌）诱导的。 P. mirabilis。脓肿来源的中性粒细胞虽然不能显着杀死已被体内吞噬的细胞内生物，但可以吞噬并杀死它们在体外暴露的生物。仅由奇异假单胞菌和麸皮诱导的脓肿引起的嗜中性粒细胞杀死该生物体的能力比体内吞噬的生物体更有效。相比之下，革兰氏阳性金黄色葡萄球菌加麸皮诱导的脓肿中的中性粒细胞在随后的体外温育中能够杀死其胞内生物，因为它们可以杀死添加的金黄色葡萄球菌。从脓肿小鼠的外周血和诱导的腹膜分泌物中分离出的嗜中性粒细胞能够在体外吞噬细胞并杀死生物，其效率高于脓肿衍生的嗜中性粒细胞。革兰氏阳性生物金黄色葡萄球菌诱导的脓肿中性粒细胞可以杀死随后吞噬的体内吞噬的生物体的机制，这与革兰氏菌组合诱导的脓肿所引起的嗜中性白细胞对细胞内生物体的相对无能为力相反-阴性细菌，未知。

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