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首页> 外文期刊>Infection and immunity >Cultivation of Ehrlichia chaffeensis in mouse embryo, Vero, BGM, and L929 cells and study of Ehrlichia-induced cytopathic effect and plaque formation.
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Cultivation of Ehrlichia chaffeensis in mouse embryo, Vero, BGM, and L929 cells and study of Ehrlichia-induced cytopathic effect and plaque formation.

机译：在小鼠胚胎，Vero，BGM和L929细胞中培养查菲埃里希氏菌，并研究埃里希氏菌引起的细胞病变作用和斑块形成。

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摘要

We successfully propagated Ehrlichia chaffeensis in mouse embryo, Vero, BGM, and L929 cells inoculated with host cell-free ehrlichiae, indicating that E. chaffeensis is capable of entry, survival, and growth in a relatively wide range of cell types derived from different species. We demonstrated rapid adaptation of E. chaffeensis in these cell lines, so that typical morulae could be detected as early as 5 days after inoculation. E. chaffeensis-induced cytopathic effect with different morphological characteristics in mouse embryo, Vero, and L929 cells. The earliest cytopathic effect appeared in untreated and irradiated mouse embryo cells at 4 days postinoculation. As the infected foci gradually expanded, the center of the foci showed necrotic cells with pyknotic nuclei and degraded morulae. E. chaffeensis caused cell lysis in untreated and irradiated L929 cells, with formation of distinct, round macroscopic plaques at 18 days postinoculation. In untreated and irradiated Vero cells, E. chaffeensis produced infected foci composed of loosely interwoven necrotic cells, spaces of detached cells, cells filled with morulae, and uninfected cells, resulting in characteristic reticular foci. Irradiated cells generally contained many large morulae and presented larger cytopathic foci. DH82 and BGM cells did not develop obvious cytopathic foci under the conditions employed. The findings reported herein offer the opportunity to study the pathogenic mechanism of cell injury by E. chaffeensis, the basis for quantification of infectious E. chaffeensis, improved approaches for recovery of ehrlichiae from human patients and tick hosts, and additional methods for cultivation of E. chaffeensis for molecular analysis.
机译：我们在无宿主埃希氏菌接种的小鼠胚胎，Vero，BGM和L929细胞中成功繁殖了恰菲埃里希氏菌，这表明恰菲埃里希氏菌能够进入，存活和在不同物种衍生的相对广泛的细胞类型中生长。我们证明了恰菲埃里希氏菌在这些细胞系中具有快速适应性，因此可以在接种后5天之内检测到典型的桑ula。 Chaffeensis大肠杆菌诱导的小鼠胚胎，Vero和L929细胞具有不同形态特征的细胞病变作用。接种后4天，最早的细胞病变作用出现在未经处理和辐射的小鼠胚胎细胞中。随着感染的病灶逐渐扩大，病灶的中心显示出坏死性细胞，带有致死性细胞核和退化的桑ula。恰菲大肠杆菌导致未处理和辐照的L929细胞裂解，接种后18天形成明显的圆形肉眼可见斑块。在未经处理和辐照的Vero细胞中，恰菲大肠杆菌产生了受感染的病灶，该病灶由松散交织的坏死细胞，分离的细胞空间，充满桑ula的细胞和未感染的细胞组成，形成了特征性的网状病灶。受辐照的细胞通常含有许多大的桑ula，并表现出较大的细胞病变灶。在所采用的条件下，DH82和BGM细胞没有形成明显的细胞病变灶。本文报道的发现为研究恰菲埃希氏菌引起的细胞损伤的致病机制提供了机会，量化了恰菲埃希菌的传染性基础，改进了从人类患者和壁虱宿主中回收埃希氏菌的方法，以及培养埃希氏菌的其他方法。 Chaffeensis用于分子分析。

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