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首页> 外文期刊>Infection and immunity >Quantitation and biological properties of released and cell-bound lipooligosaccharides from nontypeable Haemophilus influenzae.
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Quantitation and biological properties of released and cell-bound lipooligosaccharides from nontypeable Haemophilus influenzae.

机译：不可分型流感嗜血杆菌释放的和与细胞结合的脂寡糖的定量和生物学特性。

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摘要

Nontypeable Haemophilus influenzae (NTHi) is a major pathogen causing otitis media in children. NTHi releases lipooligosaccharide (LOS) as outer membrane fragments during its growth. The release of LOS may play an important role in the pathogenicity of otitis media caused by this organism. The amounts of LOS in bacterial cells and growth media for five NTHi strains were determined by quantitative silver staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These strains were estimated to have 1.6 x 10(6) to 4.8 x 10(6) LOS molecules per bacterium. During a 3-day growth period, these NTHi strains released variable but significant amounts of LOS into the growth medium. Cells started to release detectable amounts of LOS into the medium at 2 to 5 h and continued to do so for up to 48 or 72 h. The concentrations of LOS in the culture supernatants released by these five strains were 10 to 55 micrograms/ml at 24 h and 40 to 100 micrograms/ml at 72 h, which was 34 to 189% of the cell-bound LOS concentration. The biological properties of released and cell-bound LOSs from two representative strains were compared. Released LOS showed an approximately 10-fold increase in inducing human monocytes to produce tumor necrosis factor alpha, interleukin 1 beta, and interleukin 6, a 13- to 28-fold increase in mouse lethal toxicity, and a 16- to 37-fold increase in the clotting of Limulus amebocyte lysate. These results suggested that released LOS or its inflammatory mediators play a more important role than the LOS in bacteria in the pathogenicity of otitis media caused by this organism.
机译：非分型流感嗜血杆菌（NTHi）是引起儿童中耳炎的主要病原体。 NTHi在其生长过程中以外部膜片段形式释放脂寡糖（LOS）。 LOS的释放可能在由该生物体引起的中耳炎的致病性中起重要作用。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，通过定量银染色确定五个NTHi菌株在细菌细胞和生长培养基中的LOS含量。每个菌株估计这些菌株具有1.6 x 10（6）至4.8 x 10（6）LOS分子。在三天的生长期中，这些NTHi菌株向生长培养基中释放出可变但大量的LOS。细胞在2至5小时开始向培养基中释放可检测量的LOS，并持续释放长达48或72小时。这五个菌株释放的培养上清液中的LOS浓度在24小时时为10至55微克/毫升，在72小时时为40至100微克/毫升，这是细胞结合LOS浓度的34至189％。比较了两种代表性菌株释放的和与细胞结合的LOS的生物学特性。释放的LOS显示诱导人类单核细胞产生肿瘤坏死因子α，白介素1 beta和白介素6大约增加10倍，小鼠致死毒性增加13到28倍，增加16到37倍在Li变形细胞溶解物中的凝结。这些结果表明，在由该生物体引起的中耳炎的致病性中，释放的LOS或其炎性介质在细菌中比LOS更重要。

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