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首页> 外文期刊>Infection and immunity >Blood group glycolipids as epithelial cell receptors for Candida albicans.
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Blood group glycolipids as epithelial cell receptors for Candida albicans.

机译：血型糖脂作为白色念珠菌的上皮细胞受体。

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The role of glycosphingolipids as possible epithelial cell receptors for Candida albicans was examined by investigating the binding of biotinylated yeasts to lipids extracted from human buccal epithelial cells and separated on thin-layer chromatograms. Binding was visualized by the addition of 125I-streptavidin followed by autoradiography. Five C. albicans strains thought from earlier work to have a requirement for fucose-containing receptors all bound to the same three components in the lipid extract. A parallel chromatogram overlaid with biotinylated Ulex europaeus lectin, which is a fucose-binding lectin with a specificity for the H blood group antigen, showed that two of these glycosphingolipids carried this antigenic determinant. Preparations of crude and purified adhesin (a protein with a size of 15.7 kDa which lacked cysteine residues) from one of the strains also bound to these same two components. The third glycosphingolipid, which bound whole cells but neither preparation of adhesin, was recognized by Helix pomatia lectin, indicating that it contained N-acetylgalactosamine, possibly in the form of the A blood group antigen. Overlay assays with a sixth strain of C. albicans (GDH 2023) revealed a completely different binding pattern of four receptors, each of which contained N-acetylglucosamine. These results confirm earlier predictions about the receptor specificity of the strains made on the basis of adhesion inhibition studies and indicate that blood group antigens can act as epithelial cell receptors for C. albicans.
机译：通过研究生物素化酵母与从人颊上皮细胞提取并在薄层色谱图中分离的脂质的结合，来检查糖鞘脂作为白色念珠菌可能的上皮细胞受体的作用。通过加入125I-链霉亲和素，然后进行放射自显影，可见结合。五种白色念珠菌菌株在早期工作中被认为对含岩藻糖受体的要求都与脂质提取物中的相同三个成分结合。平行色谱图覆盖有生物素化的欧洲油菜素凝集素，这是一种与岩藻糖结合的凝集素，对H血型抗原具有特异性，表明其中两个糖鞘脂都带有该抗原决定簇。从其中一种菌株制备的粗制和纯化的粘附素（大小为15.7 kDa的蛋白质，缺少半胱氨酸残基）也与这两个相同的成分结合。 Helix pomatia lectin识别了结合全细胞但未制备粘附素的第三个糖鞘脂，表明它含有N-乙酰半乳糖胺，可能是A血型抗原形式。用第六株白色念珠菌菌株（GDH 2023）进行的重叠分析显示，四种受体的结合模式完全不同，每种受体均包含N-乙酰氨基葡萄糖。这些结果证实了基于粘附抑制研究对菌株的受体特异性进行的较早的预测，并表明血型抗原可以作为白色念珠菌的上皮细胞受体。

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