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首页> 外文期刊>Infection and immunity >Expression of Interleukin-6 in the Cornea in Response to Infection with Different Strains of Pseudomonas aeruginosa
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Expression of Interleukin-6 in the Cornea in Response to Infection with Different Strains of Pseudomonas aeruginosa

机译：铜绿假单胞菌感染菌株对角膜白细胞介素6表达的影响

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Strains of Pseudomonas aeruginosa causing keratitis can be either cytotoxic (6206) or invasive (6294), while a strain (Paer1) causing contact lens-induced acute red eye has been shown to be neither. In situ hybridization was used to examine the location and identity of cells expressing interleukin-6 (IL-6) mRNA in the murine cornea and changes in expression in response to infection with different strains of P. aeruginosa. The number of IL-6-positive cells was determined by image analysis. IL-6 protein levels were measured by an enzyme-linked immunosorbent assay. BALB/c mice were challenged by use of the wounded-cornea model with P. aeruginosa 6294, 6206, or Paer1 (2 × 106 CFU). At time intervals up to 24 h, postchallenge corneal tissue was probed for IL-6 mRNA. IL-6 mRNA expression was rapidly elevated in the epithelium in response to strains 6294 and 6206. At the conclusion of the experiments, infiltrating inflammatory cells also stained positively for IL-6 mRNA. In contrast, corneas challenged with strain Paer1 showed significant upregulation of IL-6 mRNA only at 4 h postchallenge. Three distinct patterns of IL-6 mRNA expression in the mouse cornea occur in response to these three ocular isolates ofP. aeruginosa. The data obtained for mRNA expression in the cornea for all three strains of P. aeruginosa correlated well with IL-6 protein analysis of whole-eye homogenates. Differences in the cytokine responses to these strains correlate with differences in the pathology associated with each strain and may offer an opportunity to develop strategies for the improved management of ocular inflammation.
机译：引起角膜炎的铜绿假单胞菌菌株可以是细胞毒性的（6206）或浸润性的（6294），而引起角膜接触镜诱导的急性红眼的菌株（Paer1）均不是。原位杂交用于检查鼠角膜中表达白介素6（IL-6）mRNA的细胞的位置和身份，以及对不同菌株 P感染的响应表达的变化。铜绿。通过图像分析确定IL-6阳性细胞的数目。通过酶联免疫吸附测定法测量IL-6蛋白水平。通过使用具有 P的受伤角膜模型攻击BALB / c小鼠。铜绿菌6294、6206或Paer1（2×10 6 CFU）。在长达24小时的时间间隔内，对攻击后角膜组织的IL-6 mRNA进行探测。响应菌株6294和6206，IL-6 mRNA表达在上皮中迅速升高。在实验结束时，浸润性炎症细胞的IL-6 mRNA也呈阳性染色。相反，仅在攻击后4小时，用Paer1菌株攻击的角膜显示出IL-6 mRNA的显着上调。响应于这三种眼部分离的 P，在小鼠角膜中出现三种不同的IL-6 mRNA表达模式。铜绿。获得的所有三种 P菌株在角膜中mRNA表达的数据。铜绿假单胞菌与全眼匀浆的IL-6蛋白分析密切相关。对这些菌株的细胞因子反应的差异与与每个菌株相关的病理学差异相关，并且可以提供机会来开发改善眼部炎症处理的策略。

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