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首页> 外文期刊>Infection and immunity >Vaccination of Cattle with Mycobacterium bovis Culture Filtrate Proteins and Interleukin-2 for Protection against Bovine Tuberculosis
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Vaccination of Cattle with Mycobacterium bovis Culture Filtrate Proteins and Interleukin-2 for Protection against Bovine Tuberculosis

机译：用牛分枝杆菌培养滤液蛋白和白介素-2接种牛，以预防牛结核病。

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In this study vaccines prepared from culture filtrate proteins (CFP) of Mycobacterium bovis and interleukin-2 (IL-2) were tested in cattle for their capacity to stimulate immune responses and to protect against an intratracheal challenge with virulent M. bovis. Nine groups of cattle were vaccinated with combinations of different doses of CFP and bovine IL-2 mixed with a monophosphoryl lipid A (MPL) adjuvant. An additional group was vaccinated withM. bovis BCG. Immune responses in CFP–IL-2-vaccinated animals differed from those seen in BCG-vaccinated animals by inducing high antigen-specific antibody responses and low levels of gamma interferon and IL-2 released from purified protein derivative-stimulated whole-blood cultures. In a concurrent experiment, additional animals were added to the high-dose CFP–IL-2, MPL control, and BCG groups and these expanded groups of animals were challenged intratracheally with virulent M. bovis. Although the lung lesion scores were significantly lower for both the CFP–IL-2-and BCG-vaccinated groups compared to the MPL control group, the overall level of protection was greatest for the BCG-vaccinated animals. There were more animals with extrathoracic spread of disease in the CFP–IL-2 group than in the other groups. While vaccination of cattle withM. bovis CFP gave an encouraging reduction in tuberculous lesions and did not induce a delayed-type hypersensitivity response to PPD, future CFP vaccines must prevent any extrathoracic spread of disease.
机译：在这项研究中，测试了牛分枝杆菌培养滤液蛋白（CFP）和白介素2（IL-2）制备的疫苗在牛中刺激免疫反应和预防气管内挑战的能力。毒力 M。牛羊。向九组牛接种了不同剂量的CFP和与单磷酰脂质A（MPL）佐剂混合的牛IL-2疫苗。另一组接种了 M。 bovis BCG。在CFP–IL-2疫苗接种的动物中，免疫反应与在BCG疫苗接种的动物中观察到的免疫反应不同，其诱导的高抗原特异性抗体反应以及从纯化的蛋白衍生物刺激的全血培养物中释放的γ-干扰素和IL-2水平低。在同时进行的实验中，将其他动物添加到大剂量CFP-IL-2，MPL对照和BCG组中，并对这些扩展的动物组进行气管内猛烈的 M攻击。牛羊。尽管与MPL对照组相比，CFP-IL-2和BCG疫苗接种组的肺损伤评分均显着降低，但BCG疫苗接种动物的总体保护水平最高。与其他组相比，CFP–IL-2组的胸外疾病传播动物更多。用 M对牛进行疫苗接种。 bovis CFP可以令人鼓舞地减少结核病灶，并且不会引起对PPD的迟发型超敏反应，未来的CFP疫苗必须预防任何胸外疾病的传播。

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