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首页> 外文期刊>Infection and immunity >Salmonella typhimurium infection in calves: delayed specific skin reactions directed against the O-antigenic polysaccharide chain.
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Salmonella typhimurium infection in calves: delayed specific skin reactions directed against the O-antigenic polysaccharide chain.

机译：小牛鼠伤寒沙门氏菌感染：针对O抗原多糖链的特定皮肤反应延迟。

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摘要

Specific delayed skin reactions developed after intradermal injection of Salmonella typhimurium lipopolysaccharide in 12-week-old calves which had been orally infected with S. typhimurium 5 weeks earlier. Uninfected calves showed no delayed skin reactions. Skin biopsies from skin swellings showed a massive infiltration of mononuclear cells in the skin of infected calves but not in those of uninfected calves. Persistence of infection in infected calves was confirmed by isolation of S. typhimurium from fecal specimens. The delayed skin reactions could be shown to be specific and directed against the O-polysaccharide chain of the lipopolysaccharide since none of the lipopolysaccharide chain of the lipopolysaccharide since none of the lipopolysaccharide preparations from a rough mutant of S. typhimurium, two strains with different O-polysaccharide chains, or lipid A elicited skin reactivity. To cause a reaction, the O-polysaccharide had to be in a macromolecular complex, since skin swellings were seen only after injections of either O-polysaccharide chains cross-linked by 1,2,3,4-diepoxybutane or octasaccharides from the O chain covalently linked to a straight 12-carbon aliphatic chain forming an artificial glycolipid. Injection of the pure octasaccharides of O-polysaccharide chains failed to elicit delayed skin reactions.
机译：皮内注射鼠伤寒沙门氏菌脂多糖于12周龄小牛经5周前口服鼠伤寒沙门氏菌感染后，出现了特定的延迟性皮肤反应。未感染的小牛没有皮肤反应延迟。皮肤肿胀的皮肤活检显示感染小牛的皮肤中大量单核细胞浸润，但未感染小牛的皮肤中没有。从粪便标本中分离出鼠伤寒沙门氏菌，证实了感染小牛的持续感染。延迟的皮肤反应可以显示为特异性的并且针对脂多糖的O-多糖链，因为脂多糖的脂多糖链均不存在，因为鼠伤寒沙门氏菌的粗突变体，两种菌株均具有不同的O -多糖链或脂质A引起皮肤反应。为了引起反应，O-多糖必须存在于大分子复合物中，因为仅在注射通过1,2,3,4-二环氧丁烷交联的O-多糖链或来自O链的八糖后才能看到皮肤肿胀共价连接至直链12碳脂族链，形成人工糖脂。 O-多糖链的纯八糖的注射未能引起延迟的皮肤反应。

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