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首页> 外文期刊>Infection and immunity >Salmonella typhimurium infection in calves: specific immune reactivity against O-antigenic polysaccharide detectable in in vitro assays.
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Salmonella typhimurium infection in calves: specific immune reactivity against O-antigenic polysaccharide detectable in in vitro assays.

机译：犊牛的沙门氏菌感染：对体外测定中可检测到的O抗原多糖的特异性免疫反应性。

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Peripheral blood lymphocytes collected from calves infected experimentally with Salmonella typhimurium (O antigens 4,5,12) or Salmonella sp. serotype dublin (O 9,12) were stimulated with various bacterial cell envelope components, and their [3H]thymidine incorporation was measured. It was found that peripheral blood lymphocytes from infected calves incorporated significantly more [3H]thymidine than peripheral blood lymphocytes from uninfected controls (P values ranged from less than 0.05 to less than 0.0005). The responder cell type was found in a B-cell-depleted and T-cell-enriched population. The Salmonella infections elicited T-cell responses against at least two cell envelope components: (i) a specific response against the O-antigenic polysaccharide chain of the lipopolysaccharide (This was evident in that a polysaccharide from S. enteritidis [O 9,12] which shares a trisaccharide structure [O antigen 12 determinant] with S. typhimurium stimulated [3H]thymidine uptake, which, although lower than in the homologous system, was significantly higher than that seen after incubation with unrelated Salmonella sp serotype thompson polysaccharide.) and (ii) a response against outer membrane proteins (porins), which are present in both S. typhimurium and Salmonella sp. serotype dublin. The experiments with peripheral blood lymphocytes from Salmonella sp. serotype dublin-infected calves gave results in excellent agreement with those obtained in S. typhimurium-infected calves.
机译：从小牛收集的外周血淋巴细胞，用沙门氏菌（o抗原4,5,12）或沙门氏菌SP在实验中被实验感染。用各种细菌细胞包络组分刺激血清型都伯林（O 9,12），并测量其[3H]胸苷掺入。发现来自受感染犊牛的外周血淋巴细胞比来自未感染对照的外周血淋巴细胞更高[3H]胸腺淋巴细胞（P值范围小于0.05至小于0.0005）。在B细胞耗尽和富含T细胞群体中发现响应者细胞类型。沙门氏菌感染引发了对至少两个细胞包络组分的T细胞应答：（i）对脂多糖的O-抗原多糖链的特异性反应（这在来自S.肠炎的多糖中是明显的[O 9,12]与S.毒蕈醋胺刺激的三糖结构[o抗原12决定簇]分享三糖结构[3H]胸苷摄取，虽然低于同源体系，但与与无关的沙门氏菌SP血清型多糖一起孵育后显着高于此。）和（ii）对外膜蛋白（孔蛋白）的反应，其存在于S.培氏菌和沙门氏菌SP中。血清型都柏林。来自沙门氏菌的外周血淋巴细胞的实验。血清型都柏林感染的小牛的结果得到了与在伤寒感染犊牛中获得的人的良好协议。

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