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首页> 外文期刊>Infection and immunity >Identification of a Streptococcus agalactiae protein antigen associated with bovine mastitis isolates.
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Identification of a Streptococcus agalactiae protein antigen associated with bovine mastitis isolates.

机译：与牛乳腺炎分离株有关的无乳链球菌蛋白抗原的鉴定。

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Immunoblotting was used to analyze the immune response of cows to Streptococcus agalactiae. Antibody from the milk of cows immunized (via the superficial inguinal lymph node) with formalinized S. agalactiae cells or from the milk of cows with S. agalactiae mastitis reacted strongly with a group of high-molecular-weight proteinaceous antigens. The two most predominant antigenic polypeptides in this group had apparent molecular weights of 97,000 and 104,000. Because the data indicated that these two antigens, as well as several minor antigens sometimes observed in the 70- to 100-kilodalton size range, seemed to be different forms of the same protein, we refer to the entire group as Sas97/104. A monoclonal antibody that was reactive with Sas97/104 was derived and was used to purify the antigen by affinity chromatography. Whole-cell and colony blot enzyme-linked immunoassays with either the monoclonal antibody or a polyclonal serum sample raised against the affinity-purified antigen indicated that this antigen (or cross-reactive proteins with higher molecular weights) is present on the S. agalactiae strains that were freshly isolated from mastitic cows. However, the antigen was not detected in S. agalactiae of human origin, bovine strains of S. agalactiae maintained for a prolonged period in the laboratory, or other streptococci. The data are consistent with the notion that Sas97/104 is a surface antigen and does not correspond to previously described type-specific antigens of group B streptococci.
机译：免疫印迹用于分析奶牛对无乳链球菌的免疫反应。用福尔马林无乳链球菌细胞免疫（通过腹股沟腹股沟淋巴结）的牛乳或无乳链球菌乳腺炎的牛乳中的抗体与一组高分子量蛋白质抗原强烈反应。该组中两个最主要的抗原多肽的表观分子量分别为97,000和104,000。因为数据表明这两种抗原以及有时在70至100千道尔顿大小范围内观察到的几种次要抗原似乎是同一蛋白的不同形式，所以我们将整个组称为Sas97 / 104。衍生了与Sas97 / 104具有反应性的单克隆抗体，并用于通过亲和色谱纯化抗原。用针对亲和纯化抗原的单克隆抗体或多克隆血清样品进行的全细胞和菌落印迹酶联免疫测定表明，该抗原（或分子量较高的交叉反应蛋白）存在于无乳链球菌菌株中是刚从乳香牛身上分离出来的。但是，在人类来源的无乳链球菌，实验室中长时间保存的无乳链球菌牛株或其他链球菌中均未检测到抗原。数据与Sas97 / 104是表面抗原且不对应于先前描述的B组链球菌的类型特异性抗原的概念一致。

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