...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Characterization of Cryptococcus neoformans capsular glucuronoxylomannan polysaccharide with monoclonal antibodies.
【24h】

Characterization of Cryptococcus neoformans capsular glucuronoxylomannan polysaccharide with monoclonal antibodies.

机译：用单克隆抗体表征新型隐球菌荚膜葡糖醛酸氧甘露聚糖多糖。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mice were immunized with Cryptococcus neoformans serotype A capsular glucuronoxylomannan (GXM) conjugated to bovine serum albumin-adipic dihydrazide. Two splenocyte fusions yielded two monoclonal antibodies (MAbs) that were highly reactive in dot enzyme immunoassay, immunofluorescence, and sandwich enzyme immunoassay. The first MAb, BD-1 [immunoglobulin G1 (kappa) [IgG1(kappa)]], was GXM-A and GXM-D specific, whereas the second MAb, BA-4 (IgM), reacted with GXM-A and GXM-B. A third MAb, CD-6 [IgG1(kappa)], originated from mice immunized with O-deacetylated GXM-C-bovine serum albumin and reacted with GXMs of all four serotypes. Two of the MAbs (CD-6 and BD-1) were further characterized with chemically modified GXMs. Removal of glucuronosyl residues completely inhibited the binding of both MAbs, implicating (1----2)-beta-glucuronic acid as a key component of the epitope. Removal of (1----2)-beta-xylosyl residues decreased reactivity to an intermediate extent. O deacetylation led to a measurable decrease but had the least inhibitory effect of the three GXM derivatives tested. The combining site for these two MAbs appears to be a complex antigenic determinant involving more than one glycosidic residue.
机译：用缀合至牛血清白蛋白-己二酰二肼的新隐球菌血清A型荚膜葡糖醛酸羟甘露聚糖（GXM）免疫小鼠。两次脾细胞融合产生了两种单克隆抗体（MAb），它们在点酶免疫测定，免疫荧光和夹心酶免疫测定中具有高度反应性。第一个MAb BD-1 [免疫球蛋白G1（kappa）[IgG1（kappa）]]具有GXM-A和GXM-D特异性，而第二个MAb BA-4（IgM）与GXM-A和GXM反应-B第三种单克隆抗体CD-6 [IgG1（kappa）]源自用O-脱乙酰化GXM-C-牛血清白蛋白免疫的小鼠，并与所有四种血清型的GXMs反应。用化学修饰的GXM进一步表征了两种单克隆抗体（CD-6和BD-1）。葡萄糖醛糖基残基的去除完全抑制了两个MAb的结合，这牵涉（1 ---- 2）-β-葡萄糖醛酸是表位的关键成分。 （1 ---- 2）-β-木糖基残基的去除将反应性降低到中等程度。 O脱乙酰基导致可测量的下降，但在所测试的三种GXM衍生物中抑制作用最小。这两个MAb的结合位点似乎是涉及多个糖苷残基的复杂抗原决定簇。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号