...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Antibody response to epitopes of chlamydial major outer membrane proteins on infectious elementary bodies and of the reduced polyacrylamide gel electrophoresis-separated form.
【24h】

Antibody response to epitopes of chlamydial major outer membrane proteins on infectious elementary bodies and of the reduced polyacrylamide gel electrophoresis-separated form.

机译：抗体对衣原体主要外膜蛋白在感染性基本体上的表位和还原型聚丙烯酰胺凝胶电泳分离形式的反应。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Approximately 60% of the outer membrane of chlamydial elementary bodies (EBs) consists of the major outer membrane protein (MOMP) that has structural and metabolic functions. The antigenic properties of MOMPs from mammalian strains of serovars 1 and 2 and an avian strain of Chlamydia psittaci were analyzed. Polyclonal-monospecific antisera (PMAs), one monoclonal antibody (MAb), and polyclonal antisera (PAs) were produced against reduced polyacrylamide gel electrophoresis-separated MOMPs and against infectious EBs. Three PMAs and the MAb, which were induced by reduced polyacrylamide gel electrophoresis-separated MOMPs, reacted strongly in Western blot (immunoblot) assays with MOMPs of serovar 1 and 2 strains as well as with that of the avian strain 6BC, and two of these PMAs reacted weakly (dilution, 1:20) with the MOMP of strain LGV-2. The third PMA and the MAb against the MOMP of the serovar 2 strain did not react with the MOMP of LGV-2. Four PAs were produced against infectious EBs of the serovar 1 strain. One of these PAs reacted with the homologous MOMP and that of the avian strain 6BC but did not recognize MOMPs of other chlamydial strains. Three of the PAs reacted with MOMPs of homologous strains only and failed to recognize MOMPs of avian, serovar 2, and LGV-2 strains. Five PAs induced against infectious EBs of the serovar strain 2 reacted only with the MOMPs of the homologous strains and failed to recognize MOMPs of other strains of chlamydiae. Consequently, MOMPs of C. psittaci strains possess genus-, species-, and serovar-specific epitopes whereby the immune response to serovar-specific epitopes of MOMP predominate when infectious EBs are used for immunization.
机译：衣原体基本体（EBs）外膜的大约60％由具有结构和代谢功能的主要外膜蛋白（MOMP）组成。分析了来自血清型1和2的哺乳动物菌株和鹦鹉热衣原体的禽类菌株的MOMPs的抗原特性。针对还原的聚丙烯酰胺凝胶电泳分离的MOMP和抗传染性EB产生了多克隆单特异性抗血清（PMA），一种单克隆抗体（MAb）和多克隆抗血清（PAs）。三种由聚丙烯酰胺凝胶电泳分离的MOMP诱导的PMA和MAb在Western blot（免疫印迹）分析中与血清型1和2菌株以及禽类6BC的MOMP强烈反应，其中两种PMA与LGV-2菌株的MOMP反应较弱（稀释度为1:20）。第三种PMA和抗血清2株MOMP的MAb不与LGV-2的MOMP反应。针对血清型1菌株的传染性EB产生了四个PA。这些PA之一与同源MOMP和禽类6BC的MOMP反应，但不识别其他衣原体菌株的MOMP。其中三个PA仅与同源菌株的MOMP反应，未能识别禽，血清2和LGV-2菌株的MOMP。抗血清型2的传染性EB诱导的5个PA仅与同源菌株的MOMP反应，而不能识别其他衣原体菌株的MOMP。因此，鹦鹉热衣原体菌株的MOMP具有属，种和血清型特异性表位，由此当使用传染性EB进行免疫时，对MOMP血清型特异性表位的免疫应答占主导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号