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首页> 外文期刊>Infection and immunity >Inhibition of Streptococcus mutans glucosyltransferase activity by antiserum to a subsequence peptide.
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Inhibition of Streptococcus mutans glucosyltransferase activity by antiserum to a subsequence peptide.

机译：通过抗血清对亚序列肽的抑制变形链球菌葡糖基转移酶活性。

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摘要

An antigenic 15-amino-acid peptide sequence (gtfB.1) from the glucosyltransferase B enzyme of the cariogenic bacterium Streptococcus mutans GS-5 was identified previously from the genetic fusion of this sequence to the B subunit of cholera toxin. The resulting chimeric protein was used to raise antiserum in rabbits. This antiserum was shown to recognize the native glucosyltransferase enzyme and to inhibit its activity. The antiserum inhibited the synthesis of water-soluble glucan by approximately 40% and the synthesis of water-insoluble glucan by greater than 90%. The antiserum was shown to partially inhibit fructosyltransferase activity as well. The ability of this antipeptide antiserum to inhibit several enzymes from S. mutans suggests that these enzymes share an epitope related to enzymatic activity.
机译：先前已经从该序列与霍乱毒素的B亚基的基因融合中鉴定了来自致癌细菌变形链球菌GS-5的葡糖基转移酶B酶的抗原性15个氨基酸的肽序列（gtfB.1）。所得的嵌合蛋白用于在兔中产生抗血清。该抗血清显示出可以识别天然的葡萄糖基转移酶并抑制其活性。该抗血清将水溶性葡聚糖的合成抑制了约40％，并且将不溶于水的葡聚糖的合成抑制了超过90％。该抗血清也显示出部分抑制果糖基转移酶活性。该抗肽抗血清抑制变形链球菌中几种酶的能力表明这些酶具有与酶活性有关的表位。

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