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首页> 外文期刊>Infection and immunity >Effects of anaerobiosis and aerobiosis on interactions of human polymorphonuclear leukocytes with the dental plaque bacteria Streptococcus mutans, Capnocytophaga ochracea, and Bacteroides gingivalis.
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Effects of anaerobiosis and aerobiosis on interactions of human polymorphonuclear leukocytes with the dental plaque bacteria Streptococcus mutans, Capnocytophaga ochracea, and Bacteroides gingivalis.

机译：厌氧菌和好氧菌对人多形核白细胞与牙菌斑变形链球菌，纳豆菌丝菌和牙龈细菌的相互作用的影响。

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Human polymorphonuclear leukocytes (PMN) were able to generate and release superoxide anions upon stimulation of Streptococcus mutans, Bacteroides gingivalis, and Capnocytophaga ochracea when incubated aerobically but not when incubated anaerobically. Lysozyme release and phagocytosis by PMN were independent of oxygen, and no difference between PMN incubated aerobically or anaerobically was observed (PMN stimulated by B. gingivalis released 7.6% total lysozyme when aerobic and 6.9% when anaerobic). There were variations in lysozyme release and phagocytosis for the three organisms, particularly for phagocytosis. B. gingivalis and C. ochracea yielded lower phagocytosis values than those for S. mutans, e.g., at 1 h 67% of the initial inoculum of S. mutans was phagocytosed (versus only 40% for B. gingivalis). Transmission electron microscopy showed that both S. mutans and B. gingivalis were internalized into classical phagolysosomes. In contrast, C. ochracea showed two forms of internalization; C. ochracea either formed a classical phagolysosome or was tightly bound in the cytoplasm with no surrounding cell membrane. Intracellular killing of S. mutans and C. ochracea was unaffected by anaerobiosis, but killing of C. ochracea was much lower than that of S. mutans (1 x 10(7) to 2 x 10(7) bacteria killed compared with 5.1 x 10(7) bacteria killed at 6 h). In contrast, a greater number of B. gingivalis was killed in the presence of oxygen (5.3 x 10(7) bacteria were killed when aerobically incubated and 1.9 x 10(7) bacteria were killed when anaerobically incubated). These results suggest that the ability to survive anaerobically may enable some bacteria to evade PMN killing; however, abnormal phagocytosis may represent a more efficient way to evade both oxygen-dependent and -independent killing mechanisms, leading to enhanced virulence of the organism.
机译：有氧培养时，人多形核白细胞（PMN）能够在刺激变形链球菌，齿状拟杆菌和齿球菌后刺激产生并释放超氧阴离子，而无氧培养则不能。 PMN的溶菌酶释放和吞噬作用与氧气无关，在需氧或厌氧条件下培养的PMN之间没有差异（有氧芽孢杆菌刺激的PMN在有氧条件下释放7.6％的总溶菌酶，在无氧条件下释放6.9％）。三种生物，特别是吞噬作用的溶菌酶释放和吞噬作用存在差异。牙龈芽孢杆菌和曲霉的吞噬作用值比变形链球菌低，例如，在1 h时，变形链球菌初始接种物的67％被吞噬（而牙龈芽孢杆菌仅为40％）。透射电子显微镜显示变形链球菌和牙龈芽孢杆菌均被内化为经典吞噬溶酶体。相比之下，鱼衣原体表现出两种形式的内在化：草衣藻或形成了经典的吞噬体，或者紧密结合在细胞质中，没有周围的细胞膜。变形链球菌和曲霉的细胞内杀伤不受厌氧菌的影响，但曲菌的杀灭作用远低于变形链球菌（1 x 10（7）至2 x 10（7）的细菌杀死，而5.1 x在6小时内杀死了10（7）个细菌。相反，在有氧条件下杀死了大量牙龈芽孢杆菌（需氧培养时杀死5.3 x 10（7）细菌，而无氧培养时杀死1.9 x 10（7）细菌）。这些结果表明，厌氧生存的能力可能使某些细菌能够逃避PMN的杀灭。然而，异常的吞噬作用可能代表了一种更有效的方法，可以逃避依赖氧和不依赖氧的杀伤机制，从而提高了生物的毒性。

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