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首页> 外文期刊>Infection and immunity >Roles of the pap- and prs-encoded adhesins in Escherichia coli adherence to human uroepithelial cells.
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Roles of the pap- and prs-encoded adhesins in Escherichia coli adherence to human uroepithelial cells.

机译：pap和prs编码的粘附素在大肠杆菌对人尿道上皮细胞的粘附中的作用。

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In this study, we reexamined the structural prerequisites for the attachment of P-fimbriated Escherichia coli to human urinary tract epithelial cells. The epithelial cells were obtained from A1P1 nonsecretor individuals, who express the globoseries of glycolipids without the ABH blood group determinants, and from A1P1 secretor individuals, who in addition express globo-A, a receptor for the prsJ96 adhesin. The wild-type E. coli strains J96, AD110, and IA2 and the recombinant clones HB101 papJ96, HB101 prsJ96, HB101 papIA2, and HB101 papAD110 were tested for binding. They expressed P fimbriae, as defined by P blood group-dependent agglutination of human erythrocytes of the globoseries, but differed in reactivity with galactose alpha 1-4galactose beta (Gal alpha 1-4Gal beta)-latex beads, isolated glycolipids of the globoseries, sheep erythrocytes, and uroepithelial cells. Three different patterns of binding were represented among the recombinant clones. HB101 papIA2 and HB101 papAD110 agglutinated sheep erythrocytes and Gal alpha 1-4Gal beta-latex beads and attached to both secretor and nonsecretor epithelial cells. HB101 prsJ96 agglutinated sheep erythrocytes, reacted poorly with Gal alpha 1-4Gal beta-latex beads, and attached to A1 secretor but not to A1 nonsecretor epithelial cells. HB101 papJ96 agglutinated Gal alpha 1-4Gal beta-latex beads but not sheep erythrocytes and attached poorly to human uroepithelial cells. The receptors relevant for adhesion were analyzed by inhibition with glycolipids in suspension. The sheep erythrocyte agglutination and attachment to secretor and nonsecretor epithelial cells of HB101 papIA2 and HB101 papAD110 were inhibited by globotetraosylceramide, while the Forssman glycolipid had no effect. The sheep erythrocyte reactivity and attachment to secretor epithelial cells of HB101 prsJ96 were inhibited by the Forssman glycolipid. These results permitted three conclusions. First, the expression of functionally active Gal alpha 1-4Gal beta-specific adhesins, as in HB101 papJ96, was not sufficient to make E. coli competent to attach to human uroepithelial cells. Attachment required P fimbriae of the papIA2 or papAD110 type. Second, the sheep erythrocyte reactivity of P-fimbriated strains could not be attributed solely to recognition of the Forssman glycolipid and may not be used to define the prsJ96-encoded phenotype. Third, the P-fimbrial adhesins which mediate secretor state-independent attachment to human uroepithelial cells recognized receptor epitopes provided by globotetraosylceramide.
机译：在这项研究中，我们重新检查了P纤维化大肠杆菌附着于人尿道上皮细胞的结构前提。上皮细胞获自表达无ABH血型决定簇的糖脂球系列的A1P1非分泌者个体，以及自表达prsJ96粘附素受体globo-A的A1P1分泌者个体。测试野生型大肠杆菌菌株J96，AD110和IA2以及重组克隆HB101 papJ96，HB101 prsJ96，HB101 papIA2和HB101 papAD110的结合。他们表达了P菌毛，定义为globoseries的人类血细胞的P血型依赖性凝集，但与半乳糖α1-4 galactose beta（Gal alpha 1-4Gal beta）-乳胶珠，globoseries的分离糖脂，绵羊红细胞和尿道上皮细胞。在重组克隆中代表了三种不同的结合方式。 HB101 papIA2和HB101 papAD110凝集了绵羊红细胞和Gal alpha 1-4Galβ-乳胶珠，并附着在分泌和非分泌上皮细胞上。 HB101 prsJ96凝集的绵羊红细胞，与Gal alpha 1-4Galβ-乳胶珠反应不良，并与A1分泌者相连，但与A1非分泌者上皮细胞无关。 HB101 papJ96凝集了Gal alpha 1-4Gal beta-latex珠，但没有凝集绵羊红细胞，并且与人尿道上皮细胞的粘附性很差。通过用悬浮液中的糖脂抑制分析与粘附有关的受体。球红四糖基神经酰胺抑制了绵羊红细胞的凝集以及对HB101 papIA2和HB101 papAD110的分泌和非分泌上皮细胞的附着，而Forssman糖脂则没有作用。 Forssman糖脂可抑制绵羊HB101 prsJ96的红细胞反应性和对分泌上皮细胞的附着。这些结果得出三个结论。首先，如在HB101 papJ96中那样，具有功能活性的Gal alpha 1-4Gal beta特异性粘附素的表达不足以使大肠杆菌能够附着于人尿道上皮细胞。附着需要papIA2或papAD110类型的P菌毛。其次，P纤维化菌株的绵羊红细胞反应性不能仅归因于Forssman糖脂的识别，而不能用于定义prsJ96编码的表型。第三，介导分泌状态独立于人尿道上皮细胞的P-膜粘附素识别由球四糖神经酰胺提供的受体表位。

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