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首页> 外文期刊>Infection and immunity >Interaction of Mycoplasma dispar with bovine alveolar macrophages.
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Interaction of Mycoplasma dispar with bovine alveolar macrophages.

机译：Dispar支原体与牛肺泡巨噬细胞的相互作用。

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The capacity to avoid phagocytosis and the activation of bovine alveolar macrophages (BAM) by encapsulated Mycoplasma dispar or purified M. dispar capsule was investigated. Encapsulated and unencapsulated M. dispar were cocultured with BAM in the presence or absence of antisera prepared against unencapsulated M. dispar or purified capsule antiserum. Unopsonized mycoplasmas resisted phagocytosis, while only anti-capsule antibodies enhanced the phagocytosis of encapsulated mycoplasmas. BAM were cultured in the presence of purified M. dispar capsule or either live or heat-killed encapsulated or unencapsulated M. dispar. These BAM were then activated with Escherichia coli endotoxin or left without further activation. The supernatants of these cultures were assayed for tumor necrosis factor, interleukin 1, and glucose consumption as indicators of macrophage activation. Tumor necrosis factor and interleukin 1 were produced by BAM stimulated with unencapsulated M. dispar but not when encapsulated M. dispar or its purified capsule was used. Similarly, glucose consumption was increased in the presence of unencapsulated M. dispar, but not when BAM were cocultured with encapsulated M. dispar or purified capsule. When BAM were treated with purified capsule or encapsulated mycoplasmas, they could not be subsequently activated by endotoxin. These results indicate that encapsulated M. dispar or purified capsule exerts an inhibitory effect on the activity of BAM and prevents the activation of these cells.
机译：研究了通过包裹的Dispar支原体或纯化的M. dispar胶囊避免吞噬作用和牛肺泡巨噬细胞（BAM）活化的能力。在存在或不存在针对未包囊的葡萄球菌或纯化的胶囊抗血清制备的抗血清的情况下，将包囊和未包囊的葡萄球菌与BAM共培养。未经调理的支原体可抵抗吞噬作用，而只有抗胶囊抗体可增强封装的支原体的吞噬作用。 BAM在纯化的Dispar胶囊或活的或热灭活的包囊或未包囊的M. dispar的存在下培养。然后将这些BAM用大肠杆菌内毒素激活或不进行进一步激活而保留。测定这些培养物的上清液中的肿瘤坏死因子，白介素1和葡萄糖消耗作为巨噬细胞活化的指标。肿瘤坏死因子和白介素1是由BAM刺激而产生的，而BAM是用未包囊的葡萄球菌刺激的，而当使用包囊的葡萄球菌或其纯化的胶囊时则不会。类似地，在未包囊的葡萄球菌存在下，葡萄糖消耗增加，但与包囊的葡萄球菌或纯化的胶囊共培养BAM时，葡萄糖消耗没有增加。当用纯化的胶囊或包被的支原体治疗BAM时，它们随后不能被内毒素激活。这些结果表明，包囊的Dispar或纯化的胶囊对BAM的活性具有抑制作用，并阻止了这些细胞的活化。

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