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首页> 外文期刊>Infection and immunity >Expression of the C3d-binding protein (CR2) from Candida albicans during experimental candidiasis as measured by lymphoblastogenesis.
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Expression of the C3d-binding protein (CR2) from Candida albicans during experimental candidiasis as measured by lymphoblastogenesis.

机译：念珠菌病期间念珠菌C3d结合蛋白（CR2）的表达，通过淋巴母细胞生成进行了测量。

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The complement C3d-binding protein (CR2) of Candida albicans has been purified by immunoaffinity chromatography, and its specificity has been characterized by immunoblotting with monoclonal antibodies to the C. albicans CR2 and the mammalian CR2. Recent studies with immunoelectron microscopy indicated that the CR2 was expressed during a systemic infection in a murine model of candidiasis. As a continuation of these observations, the immunogenicity of the C. albicans CR2 was investigated in a lymphoblastogenesis assay. Lymph node cells as well as splenic lymphocytes from mice infected subcutaneously with viable blastoconidia of C. albicans reacted to the C. albicans CR2 to a significantly greater extent than did lymphocytes from uninfected mice (P less than 0.01). The maximum stimulation of splenic lymphocytes by the purified receptor occurred at a concentration of 0.54 micrograms of protein per ml after 72 h of incubation of lymphocytes and receptor. Also, splenocytes from infected or CR2-immunized mice exhibited significantly reduced responses to the T-cell-dependent mitogen phytohemagglutinin (P less than 0.01). These data indicate that lymphocytes from infected mice respond to the C. albicans CR2 in a lymphoproliferation assay to a greater extent than do lymphocytes from uninfected mice, indicating that the CR2 is expressed in vivo.
机译：白色念珠菌的补体C3d结合蛋白（CR2）已通过免疫亲和层析纯化，其特异性已通过用抗白色念珠菌CR2和哺乳动物CR2的单克隆抗体进行免疫印迹来表征。免疫电子显微镜的最新研究表明，在念珠菌病鼠模型的全身感染中表达了CR2。作为这些观察结果的延续，在淋巴母细胞生成测定中研究了白色念珠菌CR2的免疫原性。皮下感染了白色念珠菌的活生球菌的小鼠的淋巴结细胞和脾淋巴细胞对白色念珠菌CR2的反应程度明显大于未感染小鼠的淋巴细胞（P小于0.01）。淋巴细胞和受体孵育72小时后，纯化的受体对脾淋巴细胞的最大刺激发生在每毫升0.54微克蛋白质的浓度下。同样，来自感染或CR2免疫小鼠的脾细胞对T细胞依赖性丝裂原植物血凝素的反应也显着降低（P小于0.01）。这些数据表明，与未感染小鼠的淋巴细胞相比，在淋巴细胞增殖试验中来自感染小鼠的淋巴细胞对白色念珠菌CR2的反应程度更大，表明CR2在体内表达。

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