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首页> 外文期刊>Infection and immunity >Primary structure of and immunoglobulin E response to the repeat subunit of gp15/400 from human lymphatic filarial parasites.
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Primary structure of and immunoglobulin E response to the repeat subunit of gp15/400 from human lymphatic filarial parasites.

机译：gp15 / 400的重复亚基来自人淋巴丝虫原虫的初级结构和免疫球蛋白E应答。

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We have isolated and sequenced clones encoding the repeated subunit of the surface-associated glycoprotein gp15/400 from the two nematode species predominantly responsible for lymphatic filariasis in humans: Brugia malayi and Wuchereria bancrofti. The amino acid sequence of the 15-kDa subunit, derived from the nucleotide sequence of the gene fragment from B. malayi, is identical to that previously reported for B. pahangi, whereas the derived W. bancrofti protein sequence differs in only 7 of 132 residues. The identity of the protein in the two Brugia species allowed us to use a recombinant from B. pahangi to examine the serological response of adult Indonesian subjects infected with B. malayi. The polymerase chain reaction-amplified subunit was expressed in Escherichia coli via the pDS56/RBS11 plasmid and purified by nickel-chelating chromatography. A significant proportion of individuals produced antigen-specific immunoglobulin E (IgE). This was most pronounced in the individuals with elephantiasis, with 14 of 15 showing elevated titers and a mean of 3.2 ng of specific IgE ml-1. Only 2 of 15 microfilaremic individuals possessed elevated titers of specific IgE, with a mean of 0.045 ng ml-1 for the group as a whole. Asymptomatic amicrofilaremic residents showed approximately equal numbers of responders (defined as having a value in the radioimmunoassay greater than two standard deviations above controls) and nonresponders, with a group mean of 1.2 ng of antigen-specific IgE ml-1.
机译：我们已经分离并测序了克隆，该克隆编码了两个主要负责人类淋巴丝虫病的线虫物种的表面相关糖蛋白gp15 / 400的重复亚基：马来布鲁氏菌和班氏酵母。来自马来西亚芽孢杆菌基因片段核苷酸序列的15-kDa亚基氨基酸序列与先前报道的彭亨氏芽孢杆菌相同，而衍生的W.bancrofti蛋白序列仅在132个中有7个不同残留物。在两个布鲁吉亚物种中蛋白质的身份使我们能够使用来自彭​​氏芽孢杆菌的重组体来检查感染了马来西亚芽孢杆菌的印度尼西亚成年受试者的血清学应答。聚合酶链反应扩增的亚基通过pDS56 / RBS11质粒在大肠杆菌中表达，并通过镍螯合色谱法纯化。很大一部分人产生了抗原特异性免疫球蛋白E（IgE）。这在象皮病患者中最为明显，每15例中有14例显示滴度升高，平均IgE ml-1特异性为3.2 ng。 15个微丝蛋白血症个体中只有2个具有较高的特异性IgE滴度，整个组平均为0.045 ng ml-1。无症状的微丝丝虫病患者显示出大约相等数量的应答者（定义为放射免疫分析中的值大于对照值两个标准差）和无应答者，组均值为1.2 ng抗原特异性IgE ml-1。

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