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首页> 外文期刊>Infection and immunity >Role of the physical state of Salmonella lipopolysaccharide in expression of biological and endotoxic properties.
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Role of the physical state of Salmonella lipopolysaccharide in expression of biological and endotoxic properties.

机译：沙门氏菌脂多糖的物理状态在生物学和内毒素特性表达中的作用。

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Lipopolysaccharide (LPS) extracted from three strains of Salmonella typhimurium, i.e., the rough Re mutant SL1102, the rough Ra mutant TV119, and the smooth strain SH4809, was first electrodialyzed (eLPS) and then divalent cation deprived by EDTA treatment and finally made monomeric by deoxycholate solubilization. The removal of excess detergent by extensive dialysis in the absence of mineral cations resulted in the reassociation of LPS subunits into monodisperse micelles of reduced aggregation number (dLPS) as estimated by electron microscopy and gel filtration chromatography. For all LPS chemotypes tested, the developed procedure reproducibly results in stable and clear solutions of dLPS in concentrations of up to 100 mg/ml. The dLPS and eLPS preparations possessed the same reactivity with monoclonal antibodies (MAbs) raised against different LPS domains. The 100% lethal dose in galactosamine-sensitized mice of 0.01 microgram for the smooth eLPS was from 10- to 100-fold lower than that of dLPS at 0.1 to 1.0 microgram. dLPS from both the smooth strain and the Ra mutant had a significantly reduced capacity to activate the proenzyme cascade in the Limulus amoebocyte lysate assay in comparison with the slightly reduced activity of dLPS from the Re mutant. In contrast, dLPS as well as the deoxycholate-dispersed and then diluted eLPS from the smooth strain had a higher mitogenic activity on splenocytes than eLPS. The results indicate that the biological and endotoxic properties of LPS are significantly influenced by the physical state of its aggregates in aqueous solutions. The approach developed for production of a stable and dispersed form of LPS should further assist in investigation of LPS properties and interpretation of the data of endotoxic research.
机译：从鼠伤寒沙门氏菌的三个菌株（即粗糙的Re突变体SL1102，粗糙的Ra突变体TV119和光滑的SH4809）中提取的脂多糖（LPS）首先被电渗析（eLPS），然后被EDTA处理剥夺了二价阳离子，最后变成单体通过脱氧胆酸盐增溶。如通过电子显微镜和凝胶过滤色谱法所估计的，在不存在矿物阳离子的情况下，通过广泛的透析除去过量的去污剂，导致LPS亚基重新缔合成聚集数（dLPS）减小的单分散胶束。对于所有测试的LPS化学型，开发的程序可重复产生浓度高达100 mg / ml的dLPS稳定，澄清的溶液。 dLPS和eLPS制剂与针对不同LPS域的单克隆抗体（MAb）具有相同的反应性。对于半乳糖胺致敏的小鼠，光滑eLPS的0.01％的100％致死剂量比dLPS的0.1至1.0微克的致死剂量低10到100倍。与来自Re突变体的dLPS活性略有降低相比，来自平滑菌株和Ra突变体的dLPS在Li变形细胞溶解试验中激活原酶级联的能力显着降低。相反，来自平滑菌株的dLPS以及脱氧胆酸盐分散然后稀释的eLPS对脾细胞的促有丝分裂活性高于eLPS。结果表明，LPS的生物学和内毒素特性受水溶液中其聚集体的物理状态的显着影响。开发用于产生稳定和分散形式的LPS的方法应进一步有助于LPS性质的研究和对内毒素研究数据的解释。

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