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首页> 外文期刊>Infection and immunity >A mutant toxin of Vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes.
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A mutant toxin of Vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes.

机译：副溶血性弧菌热稳定的直接溶血素突变体毒素失去了溶血活性，但保留了与红细胞结合的能力。

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摘要

A mutant toxin, R7, of thermostable direct hemolysin (TDH) with a single amino acid substitution at glycine 62 was analyzed. The hemolytic activity of R7 decreased to less than 1/1,000 of that of wild-type TDH, and its mouse lethality was undetectable. This mutant toxin, however, showed a marked inhibitory effect on hemolysis by wild-type TDH. Enzyme immunoassay and flow cytometric analysis demonstrated that R7 retained approximately 50% of the ability to bind to erythrocytes compared with that of wild-type TDH, suggesting that its inhibition of hemolysis by wild-type TDH might be due to blocking the binding sites on the erythrocyte membrane. Wild-type TDH affected the erythrocyte membrane by causing an influx of calcium and propidium iodide, while R7 showed no detectable effects of these kinds. These results suggest that hemolysis by TDH consists of at least two steps, binding and postbinding, and that R7 is likely to be a postbinding activity-deficient mutant toxin of TDH.
机译：分析了在甘氨酸62处具有单个氨基酸取代的热稳定直接溶血素（TDH）的突变毒素R7。 R7的溶血活性降低到不到野生型TDH的1 / 1,000，并且其小鼠致死性无法检测。但是，这种突变毒素对野生型TDH的溶血表现出明显的抑制作用。酶免疫法和流式细胞仪分析表明，与野生型TDH相比，R7保留了约50％的结合红细胞的能力，这表明其对野生型TDH溶血的抑制作用可能是由于阻断了TDH的结合位点。红细胞膜。野生型TDH通过引起钙和碘化丙啶的涌入而影响红细胞膜，而R7没有显示出可检测到的这类作用。这些结果表明，TDH的溶血至少包括结合和后结合两个步骤，并且R7可能是TDH的结合后活性不足的突变毒素。

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