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首页> 外文期刊>Infection and immunity >Molecular and immunological characterization of the highly conserved antigen 84 from Mycobacterium tuberculosis and Mycobacterium leprae.
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Molecular and immunological characterization of the highly conserved antigen 84 from Mycobacterium tuberculosis and Mycobacterium leprae.

机译：来自结核分枝杆菌和麻风分枝杆菌的高度保守的抗原84的分子和免疫学表征。

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Crossed immunoelectrophoresis (CIE) has been used to develop a reference system for classifying mycobacterial antigens. The subsequent use of specific antibodies allowed further determination of antigens by molecular weight. The monoclonal antibody F126-2, originally raised against a 34-kDa antigen of Mycobacterium kansasii, reacted with antigen 84 (Ag84) in the CIE reference system for Mycobacterium bovis BCG and Mycobacterium tuberculosis. To characterize Ag84, we screened a lambda gt11 gene library from M. tuberculosis with antibody F126-2 and identified the encoding gene. The corresponding Mycobacterium leprae Ag84 gene was subsequently selected from a cosmid library, using the M. tuberculosis gene as a probe. Both genes were expressed as 34-kDa proteins in Escherichia coli, and the recombinant proteins indeed corresponded to Ag84 in the CIE reference system. The derived amino acid sequences of the M. tuberculosis and M. leprae proteins showed 85% identity, which indicates that Ag84 constitutes a group of highly conserved mycobacterial antigens. Antibodies of almost 60% of lepromatous leprosy patients responded to Ag84, indicating that the protein is highly immunogenic following infection in multibacillary leprosy.
机译：交叉免疫电泳（CIE）已用于开发用于对分枝杆菌抗原进行分类的参考系统。随后使用特异性抗体使得可以通过分子量进一步确定抗原。最初针对堪萨斯分枝杆菌的34 kDa抗原产生的单克隆抗体F126-2在牛分枝杆菌BCG和结核分枝杆菌的CIE参考系统中与抗原84（Ag84）反应。为了表征Ag84，我们用抗体F126-2从结核分枝杆菌中筛选了一个gt11基因库，并鉴定了编码基因。随后使用结核分枝杆菌基因作为探针从粘粒文库中选择相应的麻风分枝杆菌Ag84基因。这两个基因在大肠杆菌中均表达为34 kDa蛋白，并且重组蛋白确实对应于CIE参考系统中的Ag84。结核分枝杆菌和麻风分枝杆菌蛋白的衍生氨基酸序列显示出85％的同一性，这表明Ag84构成了一组高度保守的分枝杆菌抗原。大约60％的麻风病麻风病人的抗体对Ag84有反应，表明该蛋白质在多杆菌性麻风病中感染后具有高度免疫原性。

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