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首页> 外文期刊>Infection and immunity >Antigenic conservation of the 15,000-dalton outer membrane lipoprotein PCP of Haemophilus influenzae and biologic activity of anti-PCP antisera.
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Antigenic conservation of the 15,000-dalton outer membrane lipoprotein PCP of Haemophilus influenzae and biologic activity of anti-PCP antisera.

机译：流感嗜血杆菌15,000道尔顿外膜脂蛋白PCP的抗原保守性和抗PCP抗血清的生物学活性。

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摘要

A gene from Haemophilus influenzae encoding an outer membrane lipoprotein of about 15,000 daltons and which comigrates with the peptidoglycan-associated lipoprotein (PAL) of H. influenzae on sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been previously reported and designated pcp gene, and its product has been designated PCP. in order to obtain specific immunologic probes for the analysis of PCP expression, cellular location, and antigenic conservation in H. influenzae, pcp was fused to the lac polylinker region of plasmid pUC19 and the hybrid gene was expressed in Escherichia coli. PCP purified from these cells was used to generate rabbit and mouse polyclonal antisera and mouse monoclonal antibody against PCP. Western immunoblot analysis with anti-PCP monoclonal antibody demonstrated that PCP is present and antigenically conserved in 30 tested strains of H. influenzae, including 27 clinical nontypeable strains. Polyclonal antiserum against PCP killed 9 of 11 clinical H. influenzae strains in a complement-mediated bactericidal assay, and bactericidal activity was additive with bactericidal activity of antisera against PAL. These results indicate that PCP is a potentially valuable component for a subunit vaccine against nontypeable H. influenzae disease, especially in combination with PAL or other components.
机译：先前已经报道过来自流感嗜血杆菌的基因，其编码约15,000道尔顿的外膜脂蛋白，并且与十二指肠硫酸钠-聚丙烯酰胺凝胶电泳上的流感嗜血杆菌的肽聚糖相关脂蛋白（PAL）相对应，并被指定为pcp基因及其产物。已被指定为PCP。为了获得用于分析流感嗜血杆菌中PCP表达，细胞定位和抗原保守性的特异性免疫探针，将pcp融合到质粒pUC19的lac多接头区域，并在大肠杆菌中表达杂合基因。从这些细胞中纯化的PCP用于产生兔和小鼠多克隆抗血清以及针对PCP的小鼠单克隆抗体。用抗PCP单克隆抗体进行的Western免疫印迹分析表明，在30株经测试的流感嗜血杆菌菌株中，包括27种临床不可分型菌株，存在PCP并在抗原上保守。抗PCP的多克隆抗血清在补体介导的杀菌试验中杀死了11株临床流感嗜血杆菌中的9株，杀菌活性与抗血清对PAL的杀菌活性相加。这些结果表明，PCP是针对不可分型流感嗜血杆菌疾病的亚单位疫苗的潜在有价值成分，尤其是与PAL或其他成分结合使用时。

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