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首页> 外文期刊>Infection and immunity >Experimental infection of newborn pigs with an attaching and effacing Escherichia coli O45:K'E65' strain.
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Experimental infection of newborn pigs with an attaching and effacing Escherichia coli O45:K'E65' strain.

机译：用附着和脱落的大肠杆菌O45：K“ E65”株对新生猪进行实验性感染。

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摘要

The ability of a nonenterotoxigenic, K88-negative porcine Escherichia coli strain of serogroup O45:K"E65" to induce attaching-effacing lesions was investigated in newborn pigs. Typical attaching-effacing lesions, characterized by intimate adherence of bacteria to mature enterocyte brush borders with effacement of the microvilli, were observed on light and electron microscopy. Bacteria were also seen in intracytoplasmic vacuoles of mature enterocytes and, in areas of heavier colonization, in the lamina propria of the intestinal mucosa. A moderate inflammatory response with mild focal ulceration of the intestinal mucosa was observed. In a sequential study, we observed that the attaching-effacing lesions were well established in the duodenum, jejunum, and ileum at 12 h postinoculation but did not develop in the cecum and colon until 24 to 48 h postinoculation, although bacteria had colonized the latter areas as early as 12 h postinoculation. Initially, bacteria were very intimately attached, with an irregular arrangement on the enterocyte apical cell membrane, and subsequently reoriented to form a typical palisade arrangement with a narrow regular gap between the bacterial cell wall and the enterocyte apical cell membrane. This phenomenon of early intimate attachment of irregularly disposed bacteria has not been reported for human enteropathogenic attaching and effacing E. coli and could represent a new and different mechanism of attachment and effacement to intestinal epithelial cells.
机译：在新生猪中研究了O45：K“ E65”血清型的非肠毒素，K88阴性猪大肠杆菌菌株诱导附着的病变的能力。在光学和电子显微镜下观察到典型的附着面病变，其特征是细菌紧密附着于成熟肠细胞刷状缘并伴有微绒毛。在成熟肠细胞的胞浆内液泡中，以及在较重定植的区域中，在肠粘膜固有层中也可见到细菌。观察到中等程度的炎症反应，肠粘膜有轻度局灶性溃疡。在一项后续研究中，我们观察到在接种后12 h，十二指肠，空肠和回肠的附着面病变已很好建立，但直到接种后24至48 h，在盲肠和结肠中才形成附着性病变，尽管细菌已经定居在后者最早在接种后12 h内。最初，细菌非常紧密地附着，在肠细胞顶细胞膜上呈不规则排列，然后重新定向以形成典型的栅状排列，在细菌细胞壁和肠细胞顶细胞膜之间具有狭窄的规则间隙。对于人类肠道致病性附着和消灭大肠杆菌，尚未报道这种不规则处置细菌的早期紧密附着现象，并且可能代表了新的和不同的附着和消融到肠上皮细胞的机制。

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