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首页> 外文期刊>Infection and immunity >Live vaccine strain of Francisella tularensis: infection and immunity in mice.
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Live vaccine strain of Francisella tularensis: infection and immunity in mice.

机译：图拉弗朗西斯菌活疫苗株：小鼠的感染和免疫。

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The live vaccine strain (LVS) of Francisella tularensis caused lethal disease in several mouse strains. Lethality depended upon the dose and route of inoculation. The lethal dose for 50% of the mice (LD50) in four of six mouse strains (A/J, BALB/cHSD, C3H/HeNHSD, and SWR/J) given an intraperitoneal (i.p.) inoculation was less than 10 CFU. For the other two strains tested, C3H/HeJ and C57BL/6J, the i.p. log LD50 was 1.5 and 2.7, respectively. Similar susceptibility was observed in mice inoculated by intravenous (i.v.) and intranasal (i.n.) routes: in all cases the LD50 was less than 1,000 CFU. Regardless of the inoculation route (i.p., i.v., or i.n.), bacteria were isolated from spleen, liver, and lungs within 3 days of introduction of bacteria; numbers of bacteria increased in these infected organs over 5 days. In contrast to the other routes of inoculation, mice injected with LVS intradermally (i.d.) survived infection: the LD50 of LVS by this route was much greater than 10(5) CFU. This difference in susceptibility was not due solely to local effects at the dermal site of inoculation, since bacteria were isolated from the spleen, liver, and lungs within 3 days by this route as well. The i.d.-infected mice were immune to an otherwise lethal i.p. challenge with as many as 10(4) CFU, and immunity could be transferred with either serum, whole spleen cells, or nonadherent spleen cells (but not Ig+ cells). A variety of infectious agents induce different disease syndromes depending on the route of entry. Francisella LVS infection in mice provides a model system for analysis of locally induced protective effector mechanisms.
机译：图拉弗朗西斯菌的活疫苗株（LVS）在几种小鼠株中引起致命性疾病。致死率取决于接种的剂量和途径。腹膜内（i.p.）接种六种小鼠品系（A / J，BALB / cHSD，C3H / HeNHSD和SWR / J）中的四种对50％小鼠（LD50）的致死剂量小于10 CFU。对于测试的其他两个菌株，C3H / HeJ和C57BL / 6J，腹腔内注射。 log LD50分别为1.5和2.7。在通过静脉内（i.v.）和鼻内（i.n.）途径接种的小鼠中观察到了相似的敏感性：在所有情况下，LD50均小于1,000 CFU。不论接种途径（腹膜内，静脉内或肠内），在引入细菌后三天内从脾脏，肝脏和肺中分离出细菌;这些受感染器官在5天内细菌数量增加。与其他接种途径相反，皮内注射i.d.的LVS小鼠幸免于感染：通过该途径LVS的LD50远大于10（5）CFU。这种敏感性的差异并非仅是由于接种真皮部位的局部作用，因为细菌也是通过这种途径在3天内从脾脏，肝脏和肺中分离出来的。被i.d.感染的小鼠对原本致命的i.p.免疫。用多达10（4）个CFU进行免疫，可以用血清，整个脾细胞或非贴壁脾细胞（而不是Ig +细胞）转移免疫力。根据进入途径的不同，多种传染原可诱发不同的疾病综合征。小鼠弗朗西斯菌LVS感染提供了用于分析局部诱导的保护性效应机制的模型系统。

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