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首页> 外文期刊>Infection and immunity >Indirect measurement of intestinal immune responses to an orally administered attenuated bacterial vaccine.
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Indirect measurement of intestinal immune responses to an orally administered attenuated bacterial vaccine.

机译：对口服减毒细菌疫苗的肠道免疫反应的间接测量。

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Intestinal fluid, saliva, circulating peripheral blood lymphocytes (PBL), and serum samples obtained from 81 human adult subjects who had been orally vaccinated with either Salmonella typhi Ty21a or one of its recombinant derivatives were examined to determine the value of indirect measurements of an antigen-specific intestinal-immunoglobulin A (IgA) response. Salivary IgA failed to provide consistent or correlative responses, and no evidence of a significant relationship was apparent with the intestinal-IgA responses. No significant correlation between the specific increase in responses in serum IgA and intestinal IgA was evident. While the magnitude of the serum IgG response significantly correlated with the intestinal-IgA response (P = 0.00064), it failed to detect 14.8% of the intestinal-IgA responses. The observation that 16.6% of the subjects had delayed serum IgA responses, with a peak occurring after day 23 compared with days 12 to 14, may have contributed to the inadequacy of the serum IgA response as a correlative indicator. The serum IgG responses in these subjects were also of a diminished magnitude. Specific IgA production by circulating PBL was found to be the most sensitive (92.6% response rate) and correlative (P = 0.00071) indicator of a specific intestinal-IgA immune response. However, its value in predicting protective efficacy is untried. These studies confirm that for the assessment of an enteric bacterial vaccine, determination of in vitro specific IgA production by circulating PBL may offer a single measurement of specific immunity which is as useful as serum and intestinal measurements combined.
机译：检查了从81例伤寒沙门氏菌Ty21a或其重组衍生物之一口服接种的人类成年受试者中获得的肠液，唾液，循环外周血淋巴细胞（PBL）和血清样本，以确定间接测量抗原的价值特异性肠道免疫球蛋白A（IgA）反应。唾液IgA无法提供一致或相关的反应，并且没有证据表明与肠IgA反应有显着关系。血清IgA和肠道IgA反应的特异性增加之间无明显相关性。尽管血清IgG应答的幅度与肠IgA应答显着相关（P = 0.00064），但未能检测到14.8％的肠IgA应答。观察到16.6％的受试者血清IgA反应延迟，与第12天到第14天相比，第23天出现峰值，这可能是导致血清IgA反应不足的一个相关指标。这些受试者的血清IgG反应也有所减少。发现通过循环PBL产生的特定IgA是特定肠道IgA免疫反应的最敏感指标（92.6％响应率）和相关指标（P = 0.00071）。但是，其在预测保护功效方面的价值尚未得到证实。这些研究证实，对于肠细菌疫苗的评估，通过循环PBL测定体外特异性IgA产量可以提供特异性免疫的单一测定，其与血清和肠道测定相结合同样有用。

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