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首页> 外文期刊>Infection and immunity >Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion.
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Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion.

机译：葡萄糖对粘附的诱导平行诱导，并且对塑料粘附的表皮葡萄球菌具有特异性的多糖抗原：与细胞间粘附功能相关的证据。

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The initial attachment and the accumulation of Staphylococcus epidermidis on polymer surfaces in multilayered cell clusters embedded in amorphous slime, which together lead to the plastic-adherent phenotype detected by the adherence assay used in this study, have been proposed to be major virulence factors of these bacteria. An antigen specific for plastic-adherent S. epidermidis strains was detected by an indirect immunofluorescence test using absorbed antiserum raised against the strongly plastic-adherent S. epidermidis 1457. A coagglutination assay was established, which allowed the quantitation of the antigen in bacterial extracts under different physiologic growth conditions. Expression of the antigen and of plastic adherence depended significantly on the presence of glucose in the growth medium. Parallel to increased plastic adherence, a 32- to 64-fold increase in the amount of the antigen was detected in bacterial extracts of cells grown in tryptone soya broth (TSB) compared with that in extracts of cells grown in TSB lacking glucose. A parallel time-dependent increase of plastic adherence and expression of the antigen was observed after stimulation by glucose of stationary-phase cultures of plastic-adherent S. epidermidis strains grown in TSB lacking glucose. The antigen consisted most probably of polysaccharide, because its immunologic reactivity was completely abolished by periodate oxidation but was resistant to protease digestion. A significant proportion of cells of plastic-adherent as compared with nonadherent S. epidermidis strains grown in TSB were located in large cell clusters exceeding 50 cells, which completely disintegrated after periodate oxidation of the cell preparations. Periodate oxidation of adherent bacterial films in situ led to release of the adherent cells from the plastic surface. These results strongly indicate a functional relation of the antigen to adherence of S. epidermidis to polymer surfaces, most probably by mediating intercellular adhesion of cells leading to accumulation in multilayered cell clusters.
机译：已提出表皮葡萄球菌在聚合物表面上的初始附着和积累在无定形粘液中嵌入的多层细胞簇中，共同导致通过本研究中使用的粘附试验检测到的塑性粘附表型，是这些病毒的主要毒力因子。菌。通过间接免疫荧光测试，使用针对强粘附塑料的表皮葡萄球菌产生的吸收的抗血清，通过间接免疫荧光测试检测了对粘附塑料的表皮葡萄球菌菌株的特异性抗原。建立了共凝集测定法，该方法可以对细菌提取物中的抗原进行定量不同的生理生长条件。抗原的表达和塑料粘附性显着取决于生长培养基中葡萄糖的存在。与增加的塑料粘附性平行，在胰蛋白so大豆肉汤（TSB）中生长的细胞的细菌提取物中检测到的抗原量与在缺乏葡萄糖的TSB中生长的细胞提取物中检测到的抗原量增加了32至64倍。在葡萄糖刺激TSB中生长的缺乏葡萄糖的可塑性粘附的表皮葡萄球菌菌株的固定相培养物受葡萄糖刺激后，观察到可塑性粘附和抗原表达的平行时间依赖性增加。该抗原很可能由多糖组成，因为它的免疫反应性被高碘酸盐氧化完全消除，但对蛋白酶消化具有抗性。与在TSB中生长的非贴壁表皮葡萄球菌菌株相比，塑料贴壁细胞中有很大比例的细胞位于超过50个细胞的大型细胞簇中，在细胞制剂的高碘酸氧化后，它们会完全分解。粘附细菌膜的高碘酸盐原位氧化导致粘附细胞从塑料表面释放。这些结果有力地表明了抗原与表皮葡萄球菌对聚合物表面的粘附的功能关系，最有可能是通过介导细胞的细胞间粘附导致在多层细胞簇中的积累。

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