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Biological activity of interleukin-2 bound to Candida albicans.

机译：IL-2的生物活性与白色念珠菌结合。

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The lymphokine interleukin-2 (IL-2), which is necessary for the generation of an optimal cell-mediated immune response, has recently been shown to have lectinlike properties, with specificity for high-mannose groups. Therefore, the ability of IL-2 to bind to the mannose-rich fungus Candida albicans was examined. Heat-killed fungi preincubated with IL-2 stimulated, in a dose-dependent manner, proliferation of the IL-2-dependent cell line CTLL20. Soluble mannan, which is rich in exposed mannose groups, inhibited binding of IL-2 to C. albicans by approximately 60%, suggesting that the lectinlike properties of IL-2 are partially responsible for its fungal binding capacity. Binding of IL-2 to fungi appeared to be reversible, as C. albicans preincubated with IL-2 stimulated CTLL20 proliferation even when the fungi and cells were separated by an 0.4-microns-pore-size membrane. The lymphoproliferative response of normal human peripheral blood mononuclear cells to C. albicans was augmented when the fungus was preincubated with IL-2. Binding of 125I-IL-2 could not be inhibited by unlabeled IL-2, suggesting the absence of high-affinity receptors on C. albicans for IL-2. While the in vivo relevance remains to be determined, these data demonstrate that IL-2 can bind to C. albicans in vitro and thereby influence the host response to this medically important fungus.
机译：淋巴因子白介素2（IL-2）是产生最佳细胞介导的免疫反应所必需的，最近被证明具有凝集素样特性，对高甘露糖基团具有特异性。因此，检查了IL-2与富含甘露糖的真菌白色念珠菌结合的能力。与IL-2预孵育的热灭活真菌以剂量依赖性方式刺激了IL-2依赖性细胞系CTLL20的增殖。富含暴露的甘露糖基团的可溶性甘露聚糖可抑制IL-2与白色念珠菌的结合约60％，这表明IL-2的凝集素样特性部分负责其真菌结合能力。 IL-2与真菌的结合似乎是可逆的，因为与IL-2预孵育的白色念珠菌刺激了CTLL20增殖，即使真菌和细胞被0.4微米孔径的膜分开了。将真菌与IL-2预孵育后，正常人外周血单个核细胞对白色念珠菌的淋巴增殖反应增强。未标记的IL-2不能抑制125I-IL-2的结合，这表明白色念珠菌对IL-2没有高亲和力受体。尽管体内的相关性尚待确定，但这些数据表明，IL-2可以在体外与白色念珠菌结合，从而影响宿主对这种医学上重要的真菌的反应。

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