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首页> 外文期刊>Infection and immunity >Invasion and intracellular survival of Bordetella bronchiseptica in mouse dendritic cells.
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Invasion and intracellular survival of Bordetella bronchiseptica in mouse dendritic cells.

机译：小鼠树突状细胞中支气管败血波氏杆菌的侵袭和细胞内存活。

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摘要

We have studied the interaction between the respiratory pathogen Bordetella bronchiseptica and murine spleen dendritic cells, important antigen-presenting cells that are found in the airway epithelium. Wild-type B. bronchiseptica 5376 attached very efficiently to dendritic cells, whereas the bvg mutant ATCC 10580, wild-type strain BB7865, and its spontaneous delta bvgS mutant BB7866 bound less efficiently. However, all tested B. bronchiseptica strains were able to invade dendritic cells and survive intracellularly for at least 72 h. These results suggest that bvg-independent or bvg-downregulated products are involved in the uptake and intracellular survival. Transmission electron microscopic analysis revealed that bacteria grew and replicated intracellularly and were present in typical phagosomes, which fused with lysosomes during the initial infection period. However, in later infection stages some bacteria seemed to escape into an unfused endocytic compartment, where individual bacteria were tightly surrounded by a membrane. The in vitro interaction of B. bronchiseptica with dendritic cells reported here may be relevant to natural infections caused by this organism that lead to chronicity or an altered immune response.
机译：我们研究了呼吸道支气管败血性博德特氏菌与鼠脾树突状细胞之间的相互作用，鼠脾树突状细胞是在气道上皮中发现的重要抗原呈递细胞。野生型支气管败血性博德特氏菌5376非常有效地附着于树突状细胞，而bvg突变体ATCC 10580，野生型菌株BB7865及其自发的δbvgS突变体BB7866的结合效率较低。然而，所有测试的支气管败血波氏杆菌菌株都能够侵入树突状细胞并在细胞内存活至少72小时。这些结果表明，bvg独立或bvg下调的产物参与摄取和细胞内存活。透射电子显微镜分析表明，细菌在细胞内生长和复制，并存在于典型的吞噬体中，吞噬体在最初的感染期与溶酶体融合。但是，在后期感染阶段，某些细菌似乎逃逸到未融合的内吞室中，在那里单个细菌被膜紧紧围绕。此处报道的支气管败血性博德特氏菌与树突状细胞的体外相互作用可能与由该生物体引起的导致慢性或免疫反应改变的自然感染有关。

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