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首页> 外文期刊>Infection and immunity >Heavy-chain isotype patterns of human antibody-secreting cells induced by Haemophilus influenzae type b conjugate vaccines in relation to age and preimmunity.
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Heavy-chain isotype patterns of human antibody-secreting cells induced by Haemophilus influenzae type b conjugate vaccines in relation to age and preimmunity.

机译：b型流感嗜血杆菌结合疫苗诱导的人抗体分泌细胞重链同种型与年龄和免疫前相关。

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The influence of preexisting immunity on the heavy-chain isotypes of circulating antibody-secreting cells (AbSC) induced by vaccination with Haemophilus influenzae type b (Hib) capsular polysaccharide (HibCP) coupled to tetanus toxoid (TT) or diphtheria toxoid (DT) and by vaccination with TT or DT alone in 51 healthy adults and 9 infants was studied. In adults, the isotypes of TT and DT AbSC were dominated by immunoglobulin G1 (IgG1) followed by IgG4 and IgA1. HibCP AbSC were dominated by the isotype IgA1 followed by (in decreasing order) IgG2, IgA2, IgM, and IgG1. The isotype distributions of TT and DT AbSC were independent of whether the toxoids were coupled to HibCP, and the isotypes of HibCP AbSC were not influenced by the nature of the carrier (TT or DT). Furthermore, the isotype distributions were unaffected by recent immunization with components of the conjugates, although this reduced the numbers of AbSC. The heavy-chain gene usage of HibCP AbSC in adults differed clearly from that in infants, which was restricted largely to the genes mu, gamma 1, and alpha 1, all lying upstream in the heavy-chain constant-region gene locus, while the usage in adults also, to different extents, involved the downstream genes gamma 2 and alpha 2. The ratio between the numbers of HibCP AbSC using heavy-chain genes from the downstream duplication unit (gamma 2, gamma 4, and alpha 2) and those using genes from the upstream duplication unit (gamma 3, gamma 1, and alpha 1) correlated with the preimmunization level of natural HibCP antibodies (r = 0.59; P = 0.00002). A possible role of natural exposure for Hib or cross-reactive bacteria on the mucosal surfaces in the shaping of the isotype response to HibCP conjugate vaccines is discussed.
机译：预先存在的免疫力对接种破伤风类毒素（TT）或白喉类毒素（DT）的b型流感嗜血杆菌（Hib）荚膜多糖（HibCP）疫苗接种后诱导的循环抗体分泌细胞（AbSC）重链同型的影响对51名健康成人和9名婴儿单独接种TT或DT疫苗进行了研究。在成人中，TT和DT AbSC的同种型以免疫球蛋白G1（IgG1）其次是IgG4和IgA1为主。 HibCP AbSC以同种型IgA1为主，其次是（按降序排列）IgG2，IgA2，IgM和IgG1。 TT和DT AbSC的同种型分布与类毒素是否与HibCP偶联无关，并且HibCP AbSC的同种型不受载体（TT或DT）的性质影响。此外，同种型分布不受最近用缀合物成分免疫的影响，尽管这减少了AbSC的数量。成人中HibCP AbSC的重链基因用法与婴儿的明显不同，后者主要限于mu，γ1和alpha 1基因，它们均位于重链恒定区基因位点的上游，而在成年人中的用法也不同程度地涉及下游基因γ2和α2。使用来自下游复制单位的重链基因（γ2，γ4和α2）的HibCP AbSC数量与那些基因之间的比率使用来自上游复制单位的基因（γ3，γ1和alpha 1）与天然HibCP抗体的免疫前水平相关（r = 0.59; P = 0.00002）。讨论了Hib或交叉反应细菌在粘膜表面自然暴露在对HibCP偶联疫苗的同种型应答形成中的可能作用。

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