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首页> 外文期刊>Infection and immunity >Structure and regulation of the HSP90 gene from the pathogenic fungus Candida albicans.
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Structure and regulation of the HSP90 gene from the pathogenic fungus Candida albicans.

机译：致病性真菌白色念珠菌HSP90基因的结构和调控。

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Candida albicans HSP90 sequences were isolated by screening cDNA and genomic libraries with a probe derived from the Saccharomyces cerevisiae homolog, HSP82, which encodes a member of the heat shock protein 90 family of molecular chaperones. Identical sequences were obtained for the 2,197-bp overlap of the cDNA and gene sequences, which were derived from C. albicans 3153A and ATCC 10261, respectively. The C. albicans HSP90 gene contained no introns, and it showed strong homology (61 to 79% identity) to HSP90 sequences from other fungi, vertebrates, and plants. The C-terminal portion of the predicted Hsp90 amino acid sequence was identical to the 47-kDa protein which is thought to be immunoprotective during C. albicans infections (R. C. Matthews, J. Med. Microbiol. 36:367-370, 1992), confirming that this protein represents the C-terminal portion of the 81-kDa Hsp90 protein. Quantitative Northern (RNA) analyses revealed that C. albicans HSP90 mRNA was heat shock inducible and that its levels changed during batch growth, with its maximum levels being reached during the mid-exponential growth phase. HSP90 mRNA levels increased transiently during the yeast-to-hyphal transition but did not correlate directly with germ tube production per se. These data do not exclude a role for Hsp90 in the dimorphic transition. Southern blotting revealed only one HSP90 locus in the diploid C. albicans genome. Repeated attempts to disrupt both alleles and generate a homozygous C. albicans delta hsp90/delta hsp90 null mutant were unsuccessful. These observations suggest the existence of a single HSP90 locus which is essential for viability in C. albicans.
机译：白色念珠菌HSP90序列是通过用源自酿酒酵母同源物HSP82的探针筛选cDNA和基因组文库而分离的，该探针编码分子伴侣伴侣的热休克蛋白90家族的成员。获得了分别来自白色念珠菌3153A和ATCC 10261的cDNA和基因序列的2,197-bp重叠的相同序列。白色念珠菌HSP90基因不含内含子，与来自其他真菌，脊椎动物和植物的HSP90序列具有很强的同源性（61％至79％的同一性）。预测的Hsp90氨基酸序列的C端部分与47-kDa蛋白相同，该蛋白被认为在白色念珠菌感染期间具有免疫保护作用（RC Matthews，J。Med。Microbiol。36：367-370，1992），证实该蛋白代表81-kDa Hsp90蛋白的C端部分。定量Northern（RNA）分析显示白色念珠菌HSP90 mRNA是热休克诱导的，并且其水平在分批生长过程中发生了变化，在中间指数生长阶段达到了最大水平。 HSP90 mRNA水平在酵母菌到菌丝的过渡过程中瞬时增加，但与胚管本身的产量没有直接关系。这些数据不排除Hsp90在双态转变中的作用。 Southern印迹显示在二倍体白色念珠菌基因组中仅一个HSP90基因座。重复尝试破坏两个等位基因并产生纯合白色念珠菌δhsp90 /δhsp90 null突变体均未成功。这些观察结果表明存在单个HSP90基因座，其对于白色念珠菌的生存力是必不可少的。

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