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Distinct characteristics of initiation of the classical and alternative complement pathways by Candida albicans.

机译：白色念珠菌引发经典和替代补体途径的不同特征。

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Candida albicans is a potent activator of the complement system. The objective of this study was to characterize factors that influence the kinetics for activation of C3 and binding of C3 fragments to C. albicans. Factors that were examined included the surface properties of the yeast and contributions of the classical and alternative complement pathways. The results showed that incubation of hydrophobic, hydrophilic, or germinating yeast cells in normal human serum (NHS) containing radiolabeled C3 led to immediate accumulation of C3 on all three cell types, although the rate of accumulation of C3 on germinating cells was lower. An examination of the sites for early C3 binding showed that classical pathway initiation led to immediate, synchronous binding over the entire cell surface. A blockade of the classical pathway by absorption of putative classical pathway initiators or by chelation of calcium limited activation to the alternative pathway. Binding of C3 solely via the alternative pathway was characterized by a significant lag in the initial binding kinetics. In the absence of classical pathway initiation, the early cellular sites for C3 binding appeared as random, asynchronous foci of C3 that appeared to expand with time. The factor(s) mediating rapid deposition of C3 that was characteristic of the classical pathway initiation was reciprocally cross-absorbed by hydrophilic and hydrophobic C. albicans but was not removed by absorption of NHS with Saccharomyces cerevisiae, encapsulated Cryptococcus neoformans, or nonencapsulated C. neoformans. Delayed binding of C3 produced by absorption of serum was largely reversed by addition to the absorbed serum of immunoglobulin G isolated from NHS, indicating a significant role for a naturally occurring anti-C. albicans immunoglobulin C. in classical pathway initiation.
机译：白色念珠菌是补体系统的有效激活剂。这项研究的目的是表征影响C3激活以及C3片段与白色念珠菌结合的动力学的因素。检查的因素包括酵母的表面特性以及经典和替代补体途径的贡献。结果表明，在含有放射性标记的C3的正常人血清（NHS）中孵育疏水，亲水或发芽酵母细胞会导致C3在所有三种细胞类型上立即积累，尽管C3在发芽细胞上的积累速率较低。对早期C3结合位点的检查表明，经典途径的启动导致整个细胞表面立即，同步结合。通过吸收假定的经典途径引发剂或通过螯合钙将经典途径阻断，从而限制了对替代途径的激活。仅通过替代途径结合C3的特征在于初始结合动力学的显着滞后。在没有经典途径起始的情况下，C3结合的早期细胞位点表现为C3的随机，异步灶，并随时间而扩展。介导C3快速沉积的因子是经典途径启动的特征，亲水性和疏水性白色念珠菌相互交叉吸收，但酿酒酵母，封装的新隐球菌或未封装的C吸收NHS并不能将其清除。新甲虫。通过将吸收自NHS的免疫球蛋白G的吸收血清添加到血清中，可以大大逆转通过吸收血清产生的C3延迟结合，这表明了天然存在的抗C的重要作用。白色途径中的白色念珠菌免疫球蛋白

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