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首页> 外文期刊>Infection and immunity >Opsonization of Cryptococcus neoformans by human anticryptococcal glucuronoxylomannan antibodies.
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Opsonization of Cryptococcus neoformans by human anticryptococcal glucuronoxylomannan antibodies.

机译：人类抗隐球菌葡糖醛酸羟甘露聚糖抗体对新型隐球菌的调理作用。

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Cryptococcal meningitis occurs in 6 to 8% of human immunodeficiency virus-infected individuals. Despite the availability of powerful antifungal agents that are active against Cryptococcus neoformans, these drugs generally fail to cure cryptococcal infections in immunocompromised hosts. Alternative approaches to prevention and therapy of cryptococcosis are urgently needed. Complement promotes phagocytosis of C. neoformans, but human antibodies to cryptococcal capsular polysaccharide have not been shown to function as complement-independent opsonins. The goal of our studies was to characterize the in vitro biological function of human antibodies to glucuronoxylomannan (GXM) from individuals immunized with a GXM-tetanus toxoid (GXM-TT) vaccine. We studied sera from nine vaccinees that manifested good serologic responses to GXM-TT. The results indicate that GXM-TT-elicited antibodies promote phagocytosis of C. neoformans by both murine J774 cells and human peripheral blood mononuclear cells (PBMCs). The two sera with the highest titers of anti-GXM immunoglobulin G2 antibodies were the most opsonic. When PBMC Fc gamma RIIa receptors were blocked, a 75% decrease in phagocytosis occurred following incubation of the PBMCs with C. neoformans opsonized with these sera. Our data indicate that, in the absence of complement, human anti-GXM-TT antibodies are opsonic and that antibodies of the immunoglobulin G2 isotype are effective opsonins.
机译：隐球菌性脑膜炎发生在6至8％的人类免疫缺陷病毒感染者中。尽管可获得对新型隐球菌有活性的强大的抗真菌剂，但这些药物通常无法治愈免疫力低下宿主中的隐球菌感染。迫切需要预防和治疗隐球菌病的替代方法。补体可促进新孢梭菌的吞噬作用，但尚未显示出针对隐球菌荚膜多糖的人抗体具有与补体无关的调理素的功能。我们研究的目的是鉴定来自用GXM-破伤风类毒素（GXM-TT）疫苗免疫的个体的人对葡糖醛酸氧甘露聚糖（GXM）抗体的体外生物学功能。我们研究了九种疫苗的血清，这些疫苗对GXM-TT表现出良好的血清学应答。结果表明，GXM-TT诱导的抗体可促进鼠类J774细胞和人外周血单核细胞（PBMC）吞噬新孢子虫。抗GXM免疫球蛋白G2抗体效价最高的两个血清是调渗的。当PBMC FcγRIIa受体被阻断时，将PBMC与用这些血清调理过的新形成梭状芽胞杆菌孵育后，吞噬作用降低了75％。我们的数据表明，在没有补体的情况下，人抗GXM-TT抗体是调理素，而免疫球蛋白G2同种型的抗体是有效的调理素。

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