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Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates.

机译:Burkholderia pseudomallei O-多糖-鞭毛蛋白结合物的结构和免疫学表征。

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摘要

The O-polysaccharide moiety of Burkholderia pseudomallei 319a lipopolysaccharide was covalently linked to flagellin protein isolated from the same strain. A glycoconjugate incorporating adipic acid dihydrazide as a spacer molecule elicited high-titer immunoglobulin G responses to both the protein and carbohydrate components of the construct. This immunoglobulin G was capable of protecting diabetic rats from challenge with a heterologous B. pseudomallei strain.
机译:Burkholderia pseudomallei 319a脂多糖的O-多糖部分与从同一菌株分离的鞭毛蛋白共价连接。掺入己二酸二酰肼作为间隔物分子的糖缀合物引起对构建体的蛋白质和碳水化合物组分的高滴度免疫球蛋白G应答。该免疫球蛋白G能够保护糖尿病大鼠免受异源假芽孢杆菌菌株的攻击。

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