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Phylogenetic Analysis of Enteroaggregative and Diffusely Adherent Escherichia coli

机译:肠聚合性和弥散性粘附大肠杆菌的系统发育分析

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The phylogenetics of the various pathotypes of diarrheagenicEscherichia coli are not completely understood. In this study, we identified several plasmid and chromosomal genes in the pathogenic enteroaggregative E. coli (EAEC) prototype strain 042 and determined the prevalence of these loci among EAEC and diffusely adherent E. coli strains. The distribution of these genes is analyzed within an evolutionary framework provided by the characterization of allelic variation in housekeeping genes via multilocus enzyme electrophoresis. Our data reveal that EAEC strains are heterogeneous with respect to chromosomal and plasmid-borne genes but that the majority harbor a member of a conserved family of virulence plasmids. Comparison of plasmid and chromosomal relatedness of strains suggests clonality of chromosomal markers and a limited transfer model of plasmid distribution.
机译:腹泻型大肠杆菌的各种致病菌的系统发育尚未完全了解。在这项研究中,我们确定了致病性肠聚集性 E中的几个质粒和染色体基因。大肠埃希氏菌(EAEC)原型菌株042,并确定了这些基因座在EAEC和弥散粘附的 E中的普遍性。大肠杆菌菌株。这些基因的分布在进化框架内进行分析,该进化框架是通过多位点酶电泳表征管家基因的等位基因变异而提供的。我们的数据表明,EAEC菌株相对于染色体和质粒携带的基因而言是异质的,但是大多数都包含有毒力质粒的保守家族成员。菌株的质粒和染色体相关性的比较表明染色体标记的克隆性和质粒分布的有限转移模型。

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