• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Identification of the Cilium Binding Epitope of theMycoplasma hyopneumoniae P97 Adhesin
【24h】

Identification of the Cilium Binding Epitope of theMycoplasma hyopneumoniae P97 Adhesin

机译：猪肺炎支原体P97黏附素的C1结合表位的鉴定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mycoplasma hyopneumoniae colonizes the swine respiratory tract at the level of ciliated cells by attaching specifically to the cilium membrane. This interaction involves an adhesin called P97; the cilium binding activity of this protein was localized to the carboxy terminus, which included two repeat regions, R1 and R2 (T. Hsu, S. Artiushin, and F. C. Minion, J. Bacteriol. 179:1317–1323, 1997). To further delineate the molecular mechanisms ofM. hyopneumoniae interactions with ciliated epithelium, we used a bank of transposon inserts in the cloned P97 gene to identify the site for cilium binding by testing the truncated gene products in an in vitro microtiter plate adherence assay. These studies showed that the cilium binding site was located in the AAKPV(E) repeat sequence of P97, referred to as the R1 repeat. For functional binding, at least seven AAKPV(E) repeats were required. The adherence-blocking monoclonal antibody F1B6 also recognized this region but required fewer AAKPV(E) repeats for recognition. We then constructed R1 region-lacZgene fusions and used the resulting R1 repeat–β-galactosidase fusion proteins in an in vitro assay to confirm the role of R1 in cilium binding. A comparison of the R1 regions of M. hyopneumoniaestrains displaying variation in cilium adherence failed to identify changes that could account for the differences in adherence shown by the strains. Thus, we concluded that other proteins, in addition to P97, must be involved in cilium adherence, possibly in combination with P97.
机译：猪肺炎支原体通过特异性附着在纤毛膜上，在纤毛细胞水平上定居在猪呼吸道。这种相互作用涉及称为P97的粘附素。该蛋白的纤毛结合活性位于羧基末端，该末端包括两个重复区域R1和R2（T。Hsu，S。Artiushin和F. C. Minion，J. Bacteriol。179：1317–1323，1997）。为了进一步描述 M的分子机制。肺炎性肺炎与纤毛上皮的相互作用，我们在克隆的P97基因中使用了转座子插入库，通过在体外微量滴定板粘附试验中检测截短的基因产物来鉴定纤毛结合的位点。这些研究表明纤毛结合位点位于P97的AAKPV（E）重复序列中，称为R1重复序列。对于功能性结合，至少需要七个AAKPV（E）重复序列。粘附阻滞性单克隆抗体F1B6也可以识别该区域，但需要较少的AAKPV（E）重复序列即可识别。然后，我们构建了R1区域- lacZ 基因融合体，并在体外测定中使用了所得的R1重复序列-β-半乳糖苷酶融合蛋白，以确认R1在纤毛结合中的作用。对 M的R1区域的比较。表现出纤毛依从性变化的猪肺炎克雷伯菌菌株未能识别出可以解释菌株显示的依从性差异的变化。因此，我们得出结论，除P97外，其他蛋白质也必须参与纤毛附着，可能与P97结合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号