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首页> 外文期刊>Infection and immunity >Mucosal Delivery of Murine Interleukin-2 (IL-2) and IL-6 by Recombinant Strains of Lactococcus lactis Coexpressing Antigen and Cytokine
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Mucosal Delivery of Murine Interleukin-2 (IL-2) and IL-6 by Recombinant Strains of Lactococcus lactis Coexpressing Antigen and Cytokine

机译：乳酸乳球菌共表达抗原和细胞因子的重组菌株对小鼠白细胞介素2（IL-2）和IL-6的粘膜递送。

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Lactococcus lactis is a nonpathogenic and noncolonizing bacterium which is being developed as a vaccine delivery vehicle for immunization by mucosal routes. To determine whether lactococci can also deliver cytokines to the immune system, we have constructed novel constitutive expression strains of L. lactis which accumulate a test antigen, tetanus toxin fragment C (TTFC), within the cytoplasmic compartment and also secrete either murine interleukin-2 (IL-2) or IL-6. When mice were immunized intranasally with various different expression strains of L. lactis, the anti-TTFC antibody titers increased more rapidly and were substantially higher in mice immunized with the bacterial strains which secreted IL-2 or IL-6 in addition to their production of TTFC. This adjuvant effect was lost when the recombinant strains of L. lactis were killed by pretreatment with mitomycin C and could therefore be attributed to the secretion of IL-2 or IL-6 by the recombinant lactococci. These results provide the first example of the use of a cytokine-secreting, noninvasive experimental bacterial vaccine vector to enhance immune responses to a coexpressed heterologous antigen and point the way to experiments which will test the possible therapeutic efficacy of this mode of cytokine delivery.
机译：乳酸乳球菌是一种非致病性和非定殖性细菌，正被开发为通过粘膜途径免疫的疫苗递送载体。为了确定乳球菌是否也可以将细胞因子传递给免疫系统，我们构建了新型的L L组成型表达菌株。乳酸在细胞质区室中积聚测试抗原，破伤风毒素片段C（TTFC），并分泌鼠白介素2（IL-2）或IL-6。当用各种不同表达株的 L对小鼠进行鼻内免疫时。乳酸，抗TTFC抗体的滴度增加更快，并且在用除了分泌TTFC之外还分泌IL-2或IL-6的细菌菌株免疫的小鼠中，其滴度明显更高。当重组株 L时，这种佐剂作用消失了。丝裂霉素C预处理可杀死乳酸菌，因此可归因于重组乳球菌分泌IL-2或IL-6。这些结果提供了使用分泌细胞因子的非侵入性实验细菌疫苗载体来增强对共表达的异源抗原的免疫应答的第一个实例，并为实验提供了方法，该方法将测试这种细胞因子递送方式的可能治疗功效。

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