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首页> 外文期刊>Infection and immunity >Complete Protection against Lethal Toxoplasma gondii Infection in Mice Immunized with a Plasmid Encoding theSAG1 Gene
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Complete Protection against Lethal Toxoplasma gondii Infection in Mice Immunized with a Plasmid Encoding theSAG1 Gene

机译：完全预防致命的弓形虫感染的小鼠编码SAG1基因的质粒感染。

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Infection with the protozoan parasite Toxoplasma gondiiis transmitted to humans from infected animals by tissue cysts and oocysts excreted by cats. Immunization with inactivated parasites or recombinant proteins has at best shown partial protection. We constructed a plasmid expressing the SAG1 surface antigen of T. gondii, p1tPASAG1, and showed that animals immunized with the plasmid produce anti-SAG1 antibodies which recognize the native SAG1. Mice immunized with p1tPASAG1 showed 80 to 100% protection against challenge with the non-cyst-producing, virulent RH isolate, compared to an 80% mortality in mice immunized with empty plasmid, which is the greatest efficacy of any vaccine against T. gondii produced so far. The SAG1 molecule was analyzed for potential cytotoxic T-lymphocyte (CTL) epitopes, and four peptides with the best fit were synthesized. The ability of the peptides to stimulate gamma interferon production by CD8+ T cells from p1tPASAG1-immunized mice was tested in an ELISPOT assay, and one new CTL epitope was identified. Adoptive transfer of CD8+ T cells from p1tPASAG1-immunized to na??ve mice showed partial protection. In conclusion, DNA vaccination with p1tPASAG1 gave effective protection in mice againstT. gondii infection and the protection could be adoptively transferred by purified CD8+ T cells.
机译：刚体弓形虫弓形虫的感染是通过被猫排泄的组织囊肿和卵囊从受感染的动物传播给人类的。灭活的寄生虫或重组蛋白的免疫最多显示出部分保护作用。我们构建了一个表达 T的SAG1表面抗原的质粒。 Gondii ，p1tPASAG1，并显示用该质粒免疫的动物产生的抗SAG1抗体可识别天然SAG1。用p1tPASAG1免疫的小鼠显示出80至100％的抗非囊性，强力RH分离株攻击的保护，相比之下，用空质粒免疫的小鼠的死亡率为80％，这是任何针对 T疫苗的最大功效。到目前为止生产的gondii 。分析了SAG1分子的潜在细胞毒性T淋巴细胞（CTL）表位，并合成了四个最合适的肽。在ELISPOT分析中测试了肽刺激p1tPASAG1免疫的小鼠的CD8 + T细胞产生γ干扰素的能力，并鉴定了一种新的CTL表位。 CD8 + T细胞从p1tPASAG1免疫原代小鼠的过继转移显示出部分保护作用。总之，p1tPASAG1的DNA疫苗接种对小鼠产生了有效的针对em的保护。纯化的CD8 + T细胞可以过继转移刚地感染和保护。

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