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首页> 外文期刊>Infection and immunity >Sialylation of Lipooligosaccharide Cores Affects Immunogenicity and Serum Resistance of Campylobacter jejuni
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Sialylation of Lipooligosaccharide Cores Affects Immunogenicity and Serum Resistance of Campylobacter jejuni

机译：脂寡糖核心的唾液酸化影响空肠弯曲菌的免疫原性和血清抗性

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Three genes involved in biosynthesis of the lipooligosaccharide (LOS) core of Campylobacter jejuni MSC57360, the type strain of the HS:1 serotype, whose structure mimics GM2ganglioside, have been cloned and characterized. Mutation of genes encoding proteins with homology to a sialyl transferase (cstII) and a putative N-acetylmannosamine synthetase (neuC1), part of the biosynthetic pathway ofN-acetylneuraminic acid (NeuNAc), have identical phenotypes. The LOS cores of these mutants display identical changes in electrophoretic mobility, loss of reactivity with cholera toxin (CT), and enhanced immunoreactivity with a hyperimmune polyclonal antiserum generated against whole cells of C. jejuni MSC57360. Loss of sialic acid in the core of the neuC1 mutant was confirmed by fast atom bombardment mass spectrometry. Mutation of a gene encoding a putative β-1,4-N-acetylgalactosaminyltransferase (Cgt) resulted in LOS cores intermediate in electrophoretic mobility between that of wild type and the mutants lacking NeuNAc, loss of reactivity with CT, and a reduced immunoreactivity with hyperimmune antiserum. Chemical analyses confirmed the loss of N-acetylgalactosamine (GalNAc) and the presence of NeuNAc in the cgt mutant. These data suggest that the Cgt enzyme is capable of transferring GalNAc to an acceptor with or without NeuNAc and that the Cst enzyme is capable of transferring NeuNAc to an acceptor with or without GalNAc. A mutant with a nonsialylated LOS core is more sensitive to the bactericidal effects of human sera than the wild type or the mutant lacking GalNAc.
机译：空肠弯曲杆菌MSC57360，其结构模仿GM 2 神经节苷脂的HS：1血清型菌株中的低聚果糖（LOS）核心的生物合成的三个基因已经被发现。克隆并鉴定。编码与唾液酸转移酶（ cstII ）和推定的 N -乙酰甘露糖胺合成酶（ neuC1 ）同源的蛋白质的基因突变N -乙酰神经氨酸（NeuNAc）的信号通路具有相同的表型。这些突变体的LOS核心在电泳迁移率，与霍乱毒素（CT）的反应性丧失以及与针对C全细胞产生的超免疫多克隆抗血清的增强的免疫反应性方面表现出相同的变化。空肠MSC57360。快速原子轰击质谱法证实了 neuC1 突变体核心中唾液酸的丢失。编码假定的β-1,4- N -乙酰半乳糖胺基转移酶（Cgt）的基因的突变导致LOS核心在电泳迁移率介于野生型和缺乏NeuNAc的突变体之间处于中间状态，失去了与CT的反应性，并降低了与超免疫抗血清的免疫反应性。化学分析证实了 c 突变体中 N -乙酰半乳糖胺（GalNAc）的丢失和NeuNAc的存在。这些数据表明，Cgt酶能够在有或没有NeuNAc的情况下将GalNAc转移到受体，并且Cst酶能够在有或没有GalNAc的情况下将NeuNAc转移到受体。具有非唾液酸化的LOS核心的突变体比野生型或缺乏GalNAc的突变体对人血清的杀菌作用更为敏感。

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