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首页> 外文期刊>Infection and immunity >Long-Range Mapping of the Streptococcus agalactiae Phylogenetic Lineage Restriction Digest Pattern Type III-3 Reveals Clustering of Virulence Genes
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Long-Range Mapping of the Streptococcus agalactiae Phylogenetic Lineage Restriction Digest Pattern Type III-3 Reveals Clustering of Virulence Genes

机译：无乳链球菌系统发生谱系限制消化模式III-3型的远程定位揭示了毒力基因的聚类

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Human isolates of serotype III Streptococcus agalactiae (group B streptococcus [GBS]) can be divided into three separate phylogenetic lineages based on analysis of the restriction digest patterns (RDPs) of chromosomal DNA. Nine DNA sequences that are present in all isolates of the RDP III-3 phylogenetic lineage, but not in the other lineages, were identified by genomic subtractive hybridization. A complete physical map of a III-3 chromosome was constructed. Six of the nine III-3-specific sequences mapped to a 340-kb Sse8387I fragment which contains or is located close to known GBS virulence genes. One of the III-3-specific probes, AW-10, encodes part of GBSi1, a group II intron that is inserted at two sites within the GBS genome. The second chromosomal site for GBSi1 was isolated, sequenced, and mapped to a location near the locus responsible for hemolysin production. These findings suggest that the genetic variation that distinguishes the RDP type III-3 strains from other serotype III strains occurs largely within localized areas of the genome containing known or putative virulence genes.
机译：根据对染色体DNA的限制性酶切图谱（RDP）的分析，人类血清型III型无乳链球菌（B群链球菌[GBS]）的人类分离株可分为三个独立的系统发育谱系。通过基因组减性杂交鉴定了存在于RDP III-3系统发生谱系的所有分离株中的九种DNA序列，而不存在于其他谱系中的九种DNA序列。绘制了III-3染色体的完整物理图谱。映射到340 KB Sse 8387I片段的9个III-3特异性序列中的6个包含或邻近已知的GBS毒力基因。 III-3-特异性探针之一，AW-10，编码GBSi1的一部分，GBSi1是一种II型内含子，被插入GBS基因组内的两个位点。 GBSi1的第二个染色体位点被分离，测序并定位在负责产生溶血素的基因座附近的位置。这些发现表明，将RDP III-3型菌株与其他III型血清型菌株区分开的遗传变异主要发生在含有已知或推定毒力基因的基因组局部区域内。

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