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首页> 外文期刊>Infection and immunity >Cloned hemolysin genes from Escherichia coli that cause urinary tract infection determine different levels of toxicity in mice.
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Cloned hemolysin genes from Escherichia coli that cause urinary tract infection determine different levels of toxicity in mice.

机译：来自大肠杆菌的克隆的溶血素基因会引起尿路感染，从而确定了小鼠中不同程度的毒性。

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After intraperitoneal injection of mice with Escherichia coli strains isolated from patients with urinary tract infections, the mortality due to hemolytic (Hly+) and nonhemolytic (Hly-) isolates was 77 and 40%, respectively. Deletion of the chromosomal hemolysin (hly) determinant in an E. coli O6:K15:H31 urinary tract infection strain led to a significant reduction in toxicity for mice, and its reintroduction on a recombinant plasmid partially restored the original toxicity. Although introduction of the cloned plasmid pHly152-encoded hly determinant into the Hly- E. coli O6 mutant strain increased toxicity by only a marginal degree, transformation with the cloned chromosomal hly determinants from two E. coli strains of serotypes O18ac:K5:H- and O75:K95:H? resulted in markedly greater toxicity, even exceeding that of the original Hly+ E. coli O6 wild-type strain.
机译：小鼠腹腔注射从泌尿道感染患者中分离的大肠杆菌菌株后，溶血（Hly +）和非溶血（Hly-）分离株的死亡率分别为77％和40％。删除大肠杆菌O6：K15：H31尿路感染菌株中的染色体溶血素（hly）决定簇可导致对小鼠的毒性显着降低，并将其重新引入重组质粒中可部分恢复原始毒性。尽管将克隆的质粒pHly152编码的hly决定簇引入Hly- E. coli O6突变株仅增加了一定程度的毒性，但是用来自两种血清型O18ac：K5：H-的E. coli菌株的克隆的染色体hly决定簇进行了转化。和O75：K95：H？导致明显更大的毒性，甚至超过了原始的Hly +大肠杆菌O6野生型菌株。

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