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首页> 外文期刊>Infection and immunity >Immunological characteristics of the glycolipid antigen of Leptospira interrogans serovar lai.
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Immunological characteristics of the glycolipid antigen of Leptospira interrogans serovar lai.

机译：问号钩端螺旋体血清型糖脂糖脂抗原的免疫学特征。

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摘要

The protective antigen (PAg), a glycolipid substance, was extracted from Leptospira interrogans serovar lai strain 017 with a chloroform-methanol-water (1:2:0.8 [vol/vol/vol]) solution and partially purified by silica gel column chromatography. The PAg was not detected by Coomassie brilliant blue staining in sodium dodecyl sulfate-polyacrylamide gel electrophoresis but was observed as a smearlike band, which corresponded to a 24- to 30-kilodalton standard protein, by silver staining. The outer envelope (OE) fraction showed the same band, suggesting that the PAg was one of the chemical components of the OE. The immunogenicity and protective activity of the PAg were compared with those of the OE. The PAg as well as the OE and whole cells was able to induce agglutinating antibody against L. interrogans. Furthermore, the immune sera exhibited opsonic activity against L. interrogans, as observed by measurement of chemical luminescence derived from reactive oxygen. The PAg exhibited protective activity in hamsters challenged with lethal doses of L. interrogans. Therefore, the antigen may be useful as a component vaccine against leptospiral infection.
机译：用氯仿-甲醇-水（1：2：0.8 [vol / vol / vol]）溶液从问号钩端螺旋体血清型017菌株中提取糖脂类保护抗原（PAg），并通过硅胶柱色谱法进行部分纯化。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中考马斯亮蓝染色未检测到PAg，而银染则观察到其为涂片状条带，相当于24-30千达尔顿标准蛋白。外壳（OE）馏分显示相同的条带，表明PAg是OE的化学成分之一。将PAg的免疫原性和保护活性与OE的进行比较。 PAg以及OE细胞和全细胞都能够诱导抗询问乳杆菌的凝集抗体。此外，如通过测量源自活性氧的化学发光所观察到的那样，该免疫血清显示出对询问虫的调理活性。 PAg在用致死剂量的问号乳酸杆菌攻击的仓鼠中表现出保护活性。因此，该抗原可用作抗钩端螺旋体感染的成分疫苗。

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