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首页> 外文期刊>Infection and immunity >Role of lipopolysaccharides in adherence of Actinobacillus pleuropneumoniae to porcine tracheal rings.
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Role of lipopolysaccharides in adherence of Actinobacillus pleuropneumoniae to porcine tracheal rings.

机译：脂多糖在胸膜肺炎放线杆菌对猪气管环的粘附中的作用。

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摘要

The ability of 17 Actinobacillus pleuropneumoniae isolates, representing serotypes 1, 2, 5, and 7, to adhere to tracheal rings maintained in culture was examined. Porcine tracheal rings were infected, and 8 h after inoculation, adherent bacterial cells were evaluated. A. pleuropneumoniae adhered to tracheal rings, and marked variations were observed between and even within serotypes, suggesting that adherence of this microorganism is not primarily related to the serotype of the isolate. No relationship was found between adherence to porcine tracheal rings and plasmid profiles, virulence in mice, hemagglutination, capsular material thickness, or whole-cell protein profiles. On the other hand, we observed that all isolates of serotypes 1 and 5 had a semirough-type lipopolysaccharide (LPS), whereas isolates of serotypes 2 and 7 had a smooth-type LPS (75%) or a semirough-type LPS (25%). Results showed that 83% of isolates with a smooth-type LPS adhered in large numbers to tracheal rings, whereas 80% of isolates with a semirough-type LPS adhered poorly (P less than 0.007). Our data indicated that the degree of adherence of A. pleuropneumoniae to porcine tracheal rings appeared to be related, at least in part, to LPS profiles. Furthermore, LPS seemed to be the adhesin of A. pleuropneumoniae, since purified LPS blocked adherence of this microorganism to porcine tracheal rings.
机译：检查了代表血清型1、2、5和7的17种胸膜肺炎放线杆菌的粘附于培养物中维持的气管环的能力。感染猪气管环，接种后8小时，评估粘附的细菌细胞。胸膜肺炎链球菌粘附于气管环，并且在血清型之间甚至在血清型内观察到明显的变化，这表明该微生物的粘附主要与分离株的血清型无关。在猪气管环的粘附和质粒图谱，小鼠的毒力，血凝，荚膜材料厚度或全细胞蛋白图谱之间未发现相关性。另一方面，我们观察到血清型1和5的所有分离物均具有半粗糙型脂多糖（LPS），而血清型2和7的分离物具有平滑型LPS（75％）或半粗糙型LPS（25 ％）。结果表明，有83％的带有平滑型LPS的分离株大量附着在气管环上，而有80％的带有半粗糙型LPS的分离株附着很差（P小于0.007）。我们的数据表明胸膜肺炎链球菌对猪气管环的粘附程度似乎与LPS谱至少部分相关。此外，LPS似乎是胸膜肺炎链球菌的粘附素，因为纯化的LPS阻止了该微生物粘附于猪气管环。

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